Dataset Information

The computation of directional selectivity in the Drosophila OFF motion pathway.

ABSTRACT: In flies, the direction of moving ON and OFF features is computed separately. T4 (ON) and T5 (OFF) are the first neurons in their respective pathways to extract a directionally selective response from their non-selective inputs. Our recent study of T4 found that the integration of offset depolarizing and hyperpolarizing inputs is critical for the generation of directional selectivity. However, T5s lack small-field inhibitory inputs, suggesting they may use a different mechanism. Here we used whole-cell recordings of T5 neurons and found a similar receptive field structure: fast depolarization and persistent, spatially offset hyperpolarization. By assaying pairwise interactions of local stimulation across the receptive field, we found no amplifying responses, only suppressive responses to the non-preferred motion direction. We then evaluated passive, biophysical models and found that a model using direct inhibition, but not the removal of excitation, can accurately predict T5 responses to a range of moving stimuli.

SUBMITTER: Gruntman E

PROVIDER: S-EPMC6917495 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Publications

The computation of directional selectivity in the <i>Drosophila</i> OFF motion pathway.

Gruntman Eyal E Romani Sandro S Reiser Michael B MB

eLife 20191211

In flies, the direction of moving ON and OFF features is computed separately. T4 (ON) and T5 (OFF) are the first neurons in their respective pathways to extract a directionally selective response from their non-selective inputs. Our recent study of T4 found that the integration of offset depolarizing and hyperpolarizing inputs is critical for the generation of directional selectivity. However, T5s lack small-field inhibitory inputs, suggesting they may use a different mechanism. Here we used who ...[more]

PMID: 31825313

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Project description:We demonstrate that, as an object moves, color and motion signals from successive, widely spaced locations are integrated, but letter and digit shapes are not. The features that integrate as an object moves match those that integrate when the eyes move but the object is stationary (spatiotopic integration). We suggest that this integration is mediated by large receptive fields gated by attention and that it occurs for surface features (motion and color) that can be summed without precise alignment but not shape features (letters or digits) that require such alignment. Rapidly alternating pairs of colors and motions were presented at several locations around a circle centered at fixation. The same two stimuli alternated at each location with the phase of the alternation reversing from one location to the next. When observers attended to only one location, the stimuli alternated in both retinal coordinates and in the attended stream: feature identification was poor. When the observer's attention shifted around the circle in synchrony with the alternation, the stimuli still alternated at each location in retinal coordinates, but now attention always selected the same color and motion, with the stimulus appearing as a single unchanging object stepping across the locations. The maximum presentation rate at which the color and motion could be reported was twice that for stationary attention, suggesting (as control experiments confirmed) object-based integration of these features. In contrast, the identification of a letter or digit alternating with a mask showed no advantage for moving attention despite the fact that moving attention accessed (within the limits of precision for attentional selection) only the target and never the mask. The masking apparently leaves partial information that cannot be integrated across locations, and we speculate that for spatially defined patterns like letters, integration across large shifts in location may be limited by problems in aligning successive samples. Our results also suggest that as attention moves, the selection of any given location (dwell time) can be as short as 50 ms, far shorter than the typical dwell time for stationary attention. Moving attention can therefore sample a brief instant of a rapidly changing stream if it passes quickly through, giving access to events that are otherwise not seen.

Dataset Information

The computation of directional selectivity in the Drosophila OFF motion pathway.

Publications

The computation of directional selectivity in the <i>Drosophila</i> OFF motion pathway.

Similar Datasets

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