Dataset Information

Unbalanced Roles of Fungal Aggressiveness and Host Cultivars in the Establishment of the Fusarium Head Blight in Bread Wheat.

ABSTRACT: Fusarium head blight (FHB), caused mainly by Fusarium graminearum, is the foremost destructive disease of cereals worldwide. Effector-like molecules produced by F. graminearum play key roles in the infection process and are assumed to be one of the essential components of the pathogen's aggressiveness. However, their nature and role in the disease are still largely misunderstood. As a mean to provide relevant information about the molecular determinism of F. graminearum aggressiveness, we surveyed three F. graminearum strains on three wheat cultivars contrasted by their susceptibility to FHB. F. graminearum strains revealed large differences in aggressiveness which were mostly unchanged when facing hosts of contrasted susceptibility, suggesting that their behavior rely on intrinsic determinants. Surveying the fungal mass progress and the mycotoxin production rate in the spikes did not evidence any simple relationship with aggressiveness differences, while clues were found through a qualitative and quantitative characterization of the three strain proteomes established in planta especially with regards to early synthesized putative effectors. Independently of the wheat cultivar, the three F. graminearum strains produced systematically the same protein set during the infection but substantial differences in their abundance enabled the categorization of fungal aggressiveness. Overall, our findings show that the contrasts in F. graminearum aggressiveness were not based on the existence of strain-specific molecules but rather on the ability of the strain to ensure their sufficient accumulation. Protein abundance variance was mostly driven by the strain genetics and part was also influenced by the host cultivar but strain by cultivar interactions were marginally detected, depicting that strain-specific protein accumulations did not depend on the host cultivar. All these data provide new knowledge on fungal aggressiveness determinants and provide a resourceful repertoire of candidate effector proteins to guide further research.

SUBMITTER: Fabre F

PROVIDER: S-EPMC6917580 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Unbalanced Roles of Fungal Aggressiveness and Host Cultivars in the Establishment of the Fusarium Head Blight in Bread Wheat.

Fabre Francis F Bormann Joerg J Urbach Serge S Roche Sylvie S Langin Thierry T Bonhomme Ludovic L

Frontiers in microbiology 20191211

Fusarium head blight (FHB), caused mainly by Fusarium graminearum, is the foremost destructive disease of cereals worldwide. Effector-like molecules produced by F. graminearum play key roles in the infection process and are assumed to be one of the essential components of the pathogen's aggressiveness. However, their nature and role in the disease are still largely misunderstood. As a mean to provide relevant information about the molecular determinism of F. graminearum aggr ...[more]

PMID: 31921038

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Project description:Fungal effectors are key determinants of disease development, promoting pathogen growth and spreading in plant tissues. In the wheat/Fusarium graminearum (Fg) interaction determining the Fusarium Head Blight disease (FHB), the molecular bases of Fg aggressiveness remain widely unknown. The aim of this work is to provide a better understanding of Fusarium graminearum aggressiveness determinism during its interaction with bread wheat. Phenotyping in controlled conditions of three fungal strains was carried out on three wheat cultivars contrasted by their susceptibility to FHB. Qualitative and quantitative characterizations of the three strain's whole proteomes expressed in planta have been performed with special interest on effectors and proteins coded by Fg aggressiveness-related genes. Considering all fusaried wheat samples, this experimentation identified 615 unique Fg proteins, including 583 identified in all samples. Furthermore, 71 putative Fg effectors were found in the identified proteins. Among all the fungal proteins quantified, nearly 76% showed significant abundance differences between the Fg strains. Two-way ANOVA were computed to evaluate the contribution of the "Strain" and "Cultivar" factors on the Fg protein abundances. Abundances that were deemed significant to each factor enabled a categorization of the proteins according to the factor(s) that drive(s) the abundance differences. This included: (i) Fg proteins whose abundance differences were specifically explained by the genetic background of the strain (Strain factor, Strain_effect proteins), (ii) Fg proteins whose abundance differences were only explained by the genetic background of the host plant (Cultivar factor, Cultivar_effect proteins), (iii) Fg proteins whose abundance differences were explained by both factors (Strain+Cultivar proteins), and (iv) Fg proteins whose abundance differences observed between strains were dependent on the host cultivar (Strain×Cultivar proteins). Considering each factor individually, a total of 139 Strain_effect proteins, 82 Cultivar_effect proteins, 117 Strain+Cultivar proteins and 2 Strain×Cultivar proteins were identified.

Project description:Background: Fusarium Head Blight (FHB) is a worldwide devastating disease of bread wheat (Triticum aestivum L.). Genetic resistance is the most effective way to control FHB and many QTL related to this trait have been mapped on the wheat genetic map. This information, however, must be refined to be more efficiently used in breeding programs and for the advance of the basic research. The objective of the present study was to in-depth analyze the QTLome of FHB resistance in bread wheat, further integrating genetic, genomic, and transcriptomic data, aiming to find candidate genes. Methods: An exhaustive bibliographic review on 76 scientific papers was carried out collecting information about QTL related to FHB resistance mapped on bread wheat. A dense genetic consensus map with 572,862 loci was generated for QTL projection. Meta-analysis could be performed on 323 QTL. Candidate gene mining was carried out within the most refined loci, containing genes that were cross-validated with publicly available transcriptional expression data of wheat under Fusarium infection. Most highlighted genes were investigated for protein evidence. Results: A total of 556 QTL were found in the literature, distributed on all sub-genomes and chromosomes of wheat. Meta-analysis generated 65 meta-QTL, and this refinement allows one to find markers more tightly linked to these regions. Candidate gene mining within the most refined meta-QTL, meta-QTL 1/chr. 3B, harvested 324 genes and transcriptional data cross-validated 10 of these genes, as responsive to FHB. One is of these genes encodes a Glycosiltransferase and the other encodes for a Cytochrome P450, and these such proteins have already been verified as being responsible for FHB resistance, but the remaining eight genes still have to be further studied, as promising loci for breeding. Conclusions: The QTLome of FHB resistance in wheat was successfully assembled and a refinement in terms of number and length of loci was obtained. The integration of the QTLome with genomic and transcriptomic data has allowed for the discovery of promising candidate genes for use in breeding programs.

Dataset Information

Unbalanced Roles of Fungal Aggressiveness and Host Cultivars in the Establishment of the Fusarium Head Blight in Bread Wheat.

Publications

Unbalanced Roles of Fungal Aggressiveness and Host Cultivars in the Establishment of the Fusarium Head Blight in Bread Wheat.

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