Ontology highlight
ABSTRACT:
SUBMITTER: Rashid R
PROVIDER: S-EPMC6917801 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Rashid Rumana R Gaglia Giorgio G Chen Yu-An YA Lin Jia-Ren JR Du Ziming Z Maliga Zoltan Z Schapiro Denis D Yapp Clarence C Muhlich Jeremy J Sokolov Artem A Sorger Peter P Santagata Sandro S
Scientific data 20191217 1
In this data descriptor, we document a dataset of multiplexed immunofluorescence images and derived single-cell measurements of immune lineage and other markers in formaldehyde-fixed and paraffin-embedded (FFPE) human tonsil and lung cancer tissue. We used tissue cyclic immunofluorescence (t-CyCIF) to generate fluorescence images which we artifact corrected using the BaSiC tool, stitched and registered using the ASHLAR algorithm, and segmented using ilastik software and MATLAB. We extracted sing ...[more]