Dataset Information

Thermo/pH Responsive Star and Linear Copolymers Containing a Cholic Acid-Derived Monomer, N-Isopropylacrylamide and Acrylic Acid: Synthesis and Solution Properties.

ABSTRACT: In this work three CTAs trithiocarbonate-type were synthesized-bifunctional (with PEG), trifunctional (with glycerol), and tetrafunctional (PERT)-and used in the controlled polymerization of 2-(acryloyloxy)ethyl cholate (CAE) via reversible addition-fragmentation chain transfer (RAFT) polymerization. The resulting macroCTAs containing a cholic acid-derived polymer were chain extended with N-isopropylacrylamide with or without acrylic acid. The thermosensitive and/or pH properties of these copolymers were studied in PBS solutions. The copolymers synthesized without poly(acrylic acid) (PAAc) were unstable above the transition temperature. Similar behavior was observed for the copolymer solutions containing PAAc (2% in feed) at lower values of pH showing a faster precipitation above the LCST. On the contrary, copolymer solutions containing PAAc showed great stability at higher pH values for a longer time period at 37 °C. Interestingly, the Dh of the aggregates ranged from 18 to 30 nm in all copolymers (with or without PAAc) below the transition temperature, although the topology and the block sequence in the chain were significantly different.

SUBMITTER: Castro-Hernandez A

PROVIDER: S-EPMC6918292 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Thermo/pH Responsive Star and Linear Copolymers Containing a Cholic Acid-Derived Monomer, <i>N</i>-Isopropylacrylamide and Acrylic Acid: Synthesis and Solution Properties.

Castro-Hernández Ana A Cortez-Lemus Norma Aidé NA

Polymers 20191111 11

In this work three CTAs trithiocarbonate-type were synthesized-bifunctional (with PEG), trifunctional (with glycerol), and tetrafunctional (PERT)-and used in the controlled polymerization of 2-(acryloyloxy)ethyl cholate (CAE) via reversible addition-fragmentation chain transfer (RAFT) polymerization. The resulting macroCTAs containing a cholic acid-derived polymer were chain extended with <i>N</i>-isopropylacrylamide with or without acrylic acid. The thermosensitive and/or pH properties of these ...[more]

PMID: 31717987

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Project description:Elastin-mimetic hybrid copolymers with an alternating molecular architecture were synthesized via the step growth polymerization of azide-functionalized, telechelic poly(tert-butyl acrylate) (PtBA) and an alkyne-terminated, valine and glycine-rich peptide with a sequence of (VPGVG)2 (VG2). The resultant hybrid copolymer, [PtBA-VG2]n, contains up to six constituent building blocks and has a polydispersity index (PDI) of ~1.9. Trifluoroacetic acid (TFA) treatment of [PtBA-VG2]n gave rise to an alternating copolymer of poly(acrylic acid) (PAA) and VG2 ([PAA-VG2]n). The modular design permits facile adjustment of the copolymer composition by varying the molecular weight of PAA (22 and 63 repeat units). Characterization by dynamic light scattering indicated that the multiblock copolymers formed discrete nanoparticles at room temperature in aqueous solution at pH 3.8, with an average diameter of 250-270 nm and a particle size distribution of 0.34 for multiblock copolymers containing PAA22 and 0.17 for those containing PAA63. Upon increasing the pH to 7.4, both types of particles were able to swell without being disintegrated, reaching an average diameter of 285-300 nm for [PAA22-VG2]n and 330-350 nm for [PAA63-VG2]n, respectively. The nanoparticles were not dissociated upon the addition of urea, further confirming their unusual stability. The nanoparticles were capable of sequestering a hydrophobic fluorescent dye (pyrene), and the critical aggregation concentration (CAC) was determined to be 1.09 × 10(-2) or 1.05 × 10(-2) mg/mL for [PAA22-VG2]n and [PAA63-VG2]n, respectively. We suggest that the multiblock copolymers form through collective H-bonding and hydrophobic interactions between the PAA and VG2 peptide units, and that the unusual stability of the multiblock nanoparticles is conferred by the multiblock architecture. These hybrid multiblock copolymers are potentially useful as pH-responsive drug delivery vehicles, with the possibility of drug loading through concerted H-bonds and hydrophobic interactions.

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Dataset Information

Thermo/pH Responsive Star and Linear Copolymers Containing a Cholic Acid-Derived Monomer, N-Isopropylacrylamide and Acrylic Acid: Synthesis and Solution Properties.

Publications

Thermo/pH Responsive Star and Linear Copolymers Containing a Cholic Acid-Derived Monomer, <i>N</i>-Isopropylacrylamide and Acrylic Acid: Synthesis and Solution Properties.

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