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Hepatic metabolomics and transcriptomics to study susceptibility to ketosis in response to prepartal nutritional management.


ABSTRACT: Background:Ketosis in dairy cows is associated with body fat mobilization during the peripartal period. Sub-clinical and clinical ketosis arise more frequently in cows that are overfed energy during the entire dry (last 50 to 45?days prior to parturition) or close-up period (last ~?28 days prepartum). Methods:A retrospective analysis was performed on 12 cows from a larger cohort that were fed a higher-energy diet [1.54 Mcal/kg of dry matter (DM); 35.9% of DM corn silage and 13% of DM ground corn] during the close-up dry period, of which 6 did not develop clinical ketosis (OVE, 0.83?mmol/L plasma hydroxybutyrate; BHB) and 6 were diagnosed with clinical ketosis (KET, 1.4?mmol/L BHB) during the first week postpartum. A whole-transcriptome bovine microarray (Agilent Technologies) and metabolomics (GC-MS, LC-MS; Metabolon® Inc.) were used to perform transcript and metabolite profiling of liver tissue harvested at -?10 days relative to parturition which allowed to establish potential associations between prepartal transcriptome/metabolome profiles and susceptibility to clinical ketosis postpartum. Results:Cows in KET had greater (P?=?0.01) overall body weight between -?2 and 1?week around parturition, but similar body condition score than OVE. Although dry matter intake (DMI) did not differ prepartum, KET cows had lower (P?

SUBMITTER: Shahzad K 

PROVIDER: S-EPMC6918647 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Hepatic metabolomics and transcriptomics to study susceptibility to ketosis in response to prepartal nutritional management.

Shahzad Khuram K   Lopreiato Vincenzo V   Liang Yusheng Y   Trevisi Erminio E   Osorio Johan S JS   Xu Chuang C   Loor Juan J JJ  

Journal of animal science and biotechnology 20191218


<h4>Background</h4>Ketosis in dairy cows is associated with body fat mobilization during the peripartal period. Sub-clinical and clinical ketosis arise more frequently in cows that are overfed energy during the entire dry (last 50 to 45 days prior to parturition) or close-up period (last ~ 28 days prepartum).<h4>Methods</h4>A retrospective analysis was performed on 12 cows from a larger cohort that were fed a higher-energy diet [1.54 Mcal/kg of dry matter (DM); 35.9% of DM corn silage and 13% of  ...[more]

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2024-08-28 | GSE267250 | GEO