A Type IV-A CRISPR-Cas System in Pseudomonas aeruginosa Mediates RNA-Guided Plasmid Interference In Vivo.
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ABSTRACT:
SUBMITTER: Crowley VM
PROVIDER: S-EPMC6919247 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Publications
A Type IV-A CRISPR-Cas System in <i>Pseudomonas aeruginosa</i> Mediates RNA-Guided Plasmid Interference <i>In Vivo</i>.
Crowley Valerie M VM Catching Adam A Taylor Hannah N HN Borges Adair L AL Metcalf Josie J Bondy-Denomy Joseph J Jackson Ryan N RN
The CRISPR journal 20191127 6
Bacteria and archaea use CRISPR-Cas adaptive immune systems to destroy complementary nucleic acids using RNAs derived from CRISPR loci. Here, we provide the first functional evidence for type IV CRISPR-Cas, demonstrating that the system from <i>Pseudomonas aeruginosa</i> strain PA83 mediates RNA-guided interference against a plasmid <i>in vivo</i>, both clearing the plasmid and inhibiting its uptake. This interference depends on the putative NTP-dependent helicase activity of Csf4/DinG. ...[more]
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