Ontology highlight
ABSTRACT:
SUBMITTER: Heintzman DR
PROVIDER: S-EPMC6919565 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Heintzman Darren R DR Campos Lillian V LV Byl Jo Ann W JAW Osheroff Neil N Dewar James M JM
Cell reports 20191001 2
Termination of DNA replication occurs when two replication forks converge upon the same stretch of DNA. Resolution of topological stress by topoisomerases is crucial for fork convergence in bacteria and viruses, but it is unclear whether similar mechanisms operate during vertebrate termination. Using Xenopus egg extracts, we show that topoisomerase II (Top2) resolves topological stress to prevent converging forks from stalling during termination. Under these conditions, stalling arises due to an ...[more]