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Characterization of a human liver reference material fit for proteomics applications.

ABSTRACT: The National Institute of Standards and Technology (NIST) is creating new, economical, qualitative reference materials and data for proteomics comparisons, benchmarking and harmonization. Here we describe a large dataset from shotgun proteomic analysis of RM 8461 Human Liver for Proteomics, a reference material being developed. Consensus identifications using multiple search engines and sample preparations demonstrate a homogeneous and fit-for-purpose material that can be incorporated into automated or manual sample preparation workflows, with the resulting data used to directly assess complete sample-to-data workflows and provide harmonization and benchmarking between laboratories and techniques. Data are available via PRIDE with identifier PXD013608.

SUBMITTER: Davis WC

PROVIDER: S-EPMC6920408 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Characterization of a human liver reference material fit for proteomics applications.

Davis W Clay WC Kilpatrick Lisa E LE Ellisor Debra L DL Neely Benjamin A BA

Scientific data 20191218 1

The National Institute of Standards and Technology (NIST) is creating new, economical, qualitative reference materials and data for proteomics comparisons, benchmarking and harmonization. Here we describe a large dataset from shotgun proteomic analysis of RM 8461 Human Liver for Proteomics, a reference material being developed. Consensus identifications using multiple search engines and sample preparations demonstrate a homogeneous and fit-for-purpose material that can be incorporated into autom ...[more]

PMID: 31852895

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Dataset Information

Characterization of a human liver reference material fit for proteomics applications.

Publications

Characterization of a human liver reference material fit for proteomics applications.

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