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Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR.


ABSTRACT: This article contains the data regarding Leishmania species identification in human and canine clinical samples from a Brazilian region endemic for Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis, using a previously developed approach involving two qPCR assays (qPCR-ML and qPCR-ama). The data are related to the article "Real-time PCR to differentiate among Leishmania (Viannia) subgenus, Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis: application on Brazilian clinical samples" [1], and include also details of clinical evaluation/diagnosis of human patients and primer sequences used in the qPCR assays. The Leishmania species has been determined in 27 canine samples and 11 human samples, exploiting HRM analysis of qPCR-ML and Cq values of qPCR-ML and qPCR-ama, as reported previously [2]. The qPCR data were in agreement with the species characterization obtained with other methods such as conventional species-specific PCR, ITS1 PCR-RFLP or DNA sequencing. Despite the limited number of clinical samples, these data are encouraging for a potential application in regions where L. (Viannia) spp., L. (L.) infantum and L. (L.) amazonensis are co-endemic.

SUBMITTER: Diotallevi A 

PROVIDER: S-EPMC6920495 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Data on the differentiation among <i>Leishmania</i> (<i>Viannia</i>) spp., <i>Leishmania</i> (<i>Leishmania</i>) <i>infantum</i> and <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> in Brazilian clinical samples using real-time PCR.

Diotallevi Aurora A   Buffi Gloria G   Ceccarelli Marcello M   Neitzke-Abreu Herintha Coeto HC   Gnutzmann Laisa Vieira LV   da Costa Lima Junior Manoel Sebastião MS   Di Domenico Alice A   De Santi Mauro M   Magnani Mauro M   Galluzzi Luca L  

Data in brief 20191130


This article contains the data regarding <i>Leishmania</i> species identification in human and canine clinical samples from a Brazilian region endemic for <i>Leishmania</i> (<i>Viannia</i>) spp., <i>Leishmania</i> (<i>Leishmania</i>) <i>infantum</i> and <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i>, using a previously developed approach involving two qPCR assays (qPCR-ML and qPCR-ama). The data are related to the article "Real-time PCR to differentiate among <i>Leishmania</i> (<i>Vian  ...[more]

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