Dataset Information

Androgenic Modulation in the Primary Ovarian Growth of the Japanese eel, Anguilla japonica.

ABSTRACT: Anguilla japonica seedling production is urgently required for eel aquaculture due to the species' severely dwindling population. This study aimed to understand androgenic modulation of the primary ovarian growth, a critical development phase in females, in this semelparous fish. Through histological analysis, primordial to primary follicle transition was observed before hormone injection, and eels injected with SPH + MT showed greater synchronous follicle development than those injected with SPH alone. An in vivo experiment revealed a positive correlation (p < 0.05, r = 0.94) between the mRNA expression of ar? and increasing gonadal somatic index (GSI) < 0.75% before SPH injection. Another positive correlation was seen between ar? expression and GSI (p < 0.05, r = 0.97) after weekly SPH injections for three weeks. fshr expression was high in the SPH + MT-injected group. Significantly high fshr mRNA levels were found after weekly MT injections for two weeks (p < 0.05), whereas the expression levels dropped after flutamide injection. ar? and ar? expressions revealed different patterns before and after SPH induction. In this study, androgen modulation was found with regard to ARs expressions during primary growth and the primordial to primary follicle transition prior to hormone induction. This modulation continuously affected fshr expression and vitellogenic development after SPH induction during ovarian growth in the Japanese eel.

SUBMITTER: Shang-Chien L

PROVIDER: S-EPMC6920518 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Androgenic Modulation in the Primary Ovarian Growth of the Japanese eel, <i>Anguilla japonica</i>.

Shang-Chien Lee L Show-Wan Lou L

Zoological studies 20190225

<i>Anguilla japonica</i> seedling production is urgently required for eel aquaculture due to the species' severely dwindling population. This study aimed to understand androgenic modulation of the primary ovarian growth, a critical development phase in females, in this semelparous fish. Through histological analysis, primordial to primary follicle transition was observed before hormone injection, and eels injected with SPH + MT showed greater synchronous follicle development than those injected ...[more]

PMID: 31966303

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Project description:Long-term (1967-2008) glass eel catches were used to investigate climatic effects on the annual recruitment of Japanese eel to Taiwan. Specifically, three prevailing hypotheses that potentially explain the annual recruitment were evaluated. Hypothesis 1: high precipitation shifts the salinity front northward, resulting in favorable spawning locations. Hypothesis 2: a southward shift of the position of the North Equatorial Current (NEC) bifurcation provides a favorable larval transport route. Hypothesis 3: ocean conditions (eddy activities and productivity) along the larval migration route influence larval survival. Results of time series regression and wavelet analyses suggest that Hypothesis 1 is not supported, as the glass eel catches exhibited a negative relationship with precipitation. Hypothesis 2 is plausible. However, the catches are correlated with the NEC bifurcation with a one-year lag. Considering the time needed for larval transport (only four to six months), the one-year lag correlation does not support the direct transport hypothesis. Hypothesis 3 is supported indirectly by the results. Significant correlations were found between catches and climate indices that affect ocean productivity and eddy activities, such as the Quasi Biennial Oscillation (QBO), North Pacific Gyre Oscillation (NPGO), Pacific Decadal Oscillation (PDO), and Western Pacific Oscillation (WPO). Wavelet analysis reveals three periodicities of eel catches: 2.7, 5.4, and 10.3 years. The interannual coherence with QBO and the Niño 3.4 region suggests that the shorter-term climate variability is modulated zonally by equatorial dynamics. The low-frequency coherence with WPO, PDO, and NPGO demonstrates the decadal modulation of meridional teleconnection via ocean-atmosphere interactions. Furthermore, WPO and QBO are linked to solar activities. These results imply that the Japanese eel recruitment may be influenced by multi-timescale climate variability. Our findings call for investigation of extra-tropical ocean dynamics that affect survival of eels during transport, in addition to the existing efforts to study the equatorial system.

Project description:Japanese eel (Anguilla japonica) constitutes one of the most important food fish in Japan; accordingly, genome sequencing and linkage mapping have been conducted for the purpose of artificial cultivation. In the next stage, integration of genomic sequences within linkage groups (LG) is required to construct higher-resolution genetic markers for quantitative trait loci mapping and selective breeding of beneficial traits in farming. In order to identify LG1-linked scaffolds from the draft genome assembly (323,776 scaffolds) reported previously, we attempted to isolate chromosomes corresponding to LG1 by flow sorting and subsequent analyses. Initially, single chromosomes were randomly collected by chromosome sorting and subjected to whole-genome amplification (WGA). A total of 60 WGA samples were screened by PCR with primers for a known LG1-linked scaffold, and five positive WGA samples were sequenced by next-generation sequencing (NGS). Following reference mapping analysis of the NGS reads, four of the five WGA samples were found to be enriched by LG1-linked sequences. These samples were cytogenetically assigned to chromosome 5 by fluorescence in situ hybridization. Using blastn searches with 82,081 contigs constructed from the NGS reads of the four WGA samples as queries, 2323 scaffolds were identified as putative LG1-linked scaffolds from the draft genome assembly. The total length of the putative LG1-linked scaffolds was 99.0 Mb, comparable to the estimated DNA amounts of chromosome 5 (91.1 Mb). These results suggest that the methodology developed herein is applicable to isolate specific chromosome DNAs and integrate unanchored scaffold sequences onto a particular LG and chromosome even in teleost fishes, in which isolation of specific chromosomes by flow sorting is generally difficult owing to similar morphologies, sizes, and GC-contents among chromosomes in the genome. The putative LG1-linked scaffolds of Japanese eel contain a total of 6833 short tandem repeats which will be available for higher-resolution linkage mapping.

Dataset Information

Androgenic Modulation in the Primary Ovarian Growth of the Japanese eel, Anguilla japonica.

Publications

Androgenic Modulation in the Primary Ovarian Growth of the Japanese eel, <i>Anguilla japonica</i>.

Similar Datasets

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