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Biochemical Characterization of a Novel ?/?-Hydrolase/FSH from the White Shrimp Litopenaeus vannamei.

ABSTRACT: (1) Background: Lipases and esterases are important enzymes that share the ?/? hydrolase fold. The activity and cellular localization are important characteristics to understand the role of such enzymes in an organism. (2) Methods: Bioinformatic and biochemical tools were used to describe a new ?/? hydrolase from a Litopenaeus vannamei transcriptome (LvFHS for Family Serine Hydrolase). (3) Results: The enzyme was obtained by heterologous overexpression in Escherichia coli and showed hydrolytic activity towards short-chain lipid substrates and high affinity to long-chain lipid substrates. Anti-LvFHS antibodies were produced in rabbit that immunodetected the LvFSH enzyme in several shrimp tissues. (4) Conclusions: The protein obtained and analyzed was an ?/? hydrolase with esterase and lipase-type activity towards long-chain substrates up to 12 carbons; its immunodetection in shrimp tissues suggests that it has an intracellular localization, and predicted roles in energy mobilization and signal transduction.

SUBMITTER: Garcia-Orozco KD 

PROVIDER: S-EPMC6921030 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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(1) Background: Lipases and esterases are important enzymes that share the α/β hydrolase fold. The activity and cellular localization are important characteristics to understand the role of such enzymes in an organism. (2) Methods: Bioinformatic and biochemical tools were used to describe a new α/β hydrolase from a <i>Litopenaeus vannamei</i> transcriptome (LvFHS for Family Serine Hydrolase). (3) Results: The enzyme was obtained by heterologous overexpression in <i>Escherichia coli</i> and showe  ...[more]

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