KRas^G12D expression in the bone marrow vascular niche affects hematopoiesis with inflammatory signals.

ABSTRACT: The bone marrow (BM) niche is an important milieu where hematopoietic stem and progenitor cells (HSPCs) are maintained. Previous studies have indicated that genetic mutations in various components of the niche can affect hematopoiesis and promote hematologic abnormalities, but the impact of abnormal BM endothelial cells (BMECs), a crucial niche component, on hematopoiesis remains incompletely understood. To dissect how genetic alterations in BMECs could affect hematopoiesis, we have employed a novel inducible Tie2-CreERT2 mouse model, with a tdTomato fluorescent reporter, to introduce an oncogenic KRas^G12D mutation specifically in the adult endothelial cells. Tie2-CreERT2;KRas^G12D mice had significantly more leukocytes and myeloid cells in the blood with mostly normal

SUBMITTER: Hochstetler CL 

PROVIDER: S-EPMC6921092 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature