Dataset Information

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias.

ABSTRACT:

Background

Feline leukemia virus (FeLV) is a serious viral infection in cats. FeLV is found in some tissues, such as spleen, lymph nodes and epithelial tissues. However, there is controversy about the organ in which the virus can be reliably detected in infected cats. The purpose of this study was to determine the level of viral infection in hemolymphatic tissues, including blood, bone marrow and spleen by reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR).

Results

A total of 31 cats with clinical signs of FeLV infection associated with at least a single lineage hematologic cytopenia were included in this study. Peripheral blood, bone marrow and spleen samples were obtained from each cat. Complete blood counts, biochemical tests, and a rapid test to detect FeLV p27 antigen in blood samples of cats were performed. Of 31 cats, 9 had anemia alone, 4 had thrombocytopenia alone, 2 had neutropenia alone, 9 had bicytopenia of anemia and thrombocytopenia, 3 had bicytopenia of anemia and neutropenia, and 4 had pancytopenia. FeLV RNA was then detected by RT-qPCR in the whole blood, bone marrow and spleen. Viral RNA copy numbers were detected in all cats by RT-qPCR whereas 24 out of 31 cats were positive for the serum FeLV antigen. We detected a significantly greater number of viral RNA in the spleen compared with the whole blood and bone marrow.

Conclusion

Spleen is a site where FeLV is most frequently detected in cats with hematologic cytopenias.

SUBMITTER: Abdollahi-Pirbazari M

PROVIDER: S-EPMC6924046 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Publications

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias.

Abdollahi-Pirbazari Mehdi M Jamshidi Shahram S Nassiri Seyed Mahdi SM Zamani-Ahmadmahmudi Mohamad M

BMC veterinary research 20191219 1

<h4>Background</h4>Feline leukemia virus (FeLV) is a serious viral infection in cats. FeLV is found in some tissues, such as spleen, lymph nodes and epithelial tissues. However, there is controversy about the organ in which the virus can be reliably detected in infected cats. The purpose of this study was to determine the level of viral infection in hemolymphatic tissues, including blood, bone marrow and spleen by reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR).<h4>Results ...[more]

PMID: 31856815

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Dataset Information

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias.

Background

Results

Conclusion

Publications

Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias.

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