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Nitrene Transfer Catalyzed by a Non-Heme Iron Enzyme and Enhanced by Non-Native Small-Molecule Ligands.

ABSTRACT: Transition-metal catalysis is a powerful tool for the construction of chemical bonds. Here we show that Pseudomonas savastanoi ethylene-forming enzyme, a non-heme iron enzyme, can catalyze olefin aziridination and nitrene C-H insertion, and that these activities can be improved by directed evolution. The non-heme iron center allows for facile modification of the primary coordination sphere by addition of metal-coordinating molecules, enabling control over enzyme activity and selectivity using small molecules.

SUBMITTER: Goldberg NW

PROVIDER: S-EPMC6924163 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Nitrene Transfer Catalyzed by a Non-Heme Iron Enzyme and Enhanced by Non-Native Small-Molecule Ligands.

Goldberg Nathaniel W NW Knight Anders M AM Zhang Ruijie K RK Arnold Frances H FH

Journal of the American Chemical Society 20191206 50

Transition-metal catalysis is a powerful tool for the construction of chemical bonds. Here we show that <i>Pseudomonas savastanoi</i> ethylene-forming enzyme, a non-heme iron enzyme, can catalyze olefin aziridination and nitrene C-H insertion, and that these activities can be improved by directed evolution. The non-heme iron center allows for facile modification of the primary coordination sphere by addition of metal-coordinating molecules, enabling control over enzyme activity and selectivity u ...[more]

PMID: 31790588

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