Active role of elongation factor G in maintaining the mRNA reading frame during translation.
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ABSTRACT:
SUBMITTER: Peng BZ
PROVIDER: S-EPMC6924986 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Publications
Active role of elongation factor G in maintaining the mRNA reading frame during translation.
Peng Bee-Zen BZ Bock Lars V LV Belardinelli Riccardo R Peske Frank F Grubmüller Helmut H Rodnina Marina V MV
Science advances 20191220 12
During translation, the ribosome moves along the mRNA one codon at a time with the help of elongation factor G (EF-G). Spontaneous changes in the translational reading frame are extremely rare, yet how the precise triplet-wise step is maintained is not clear. Here, we show that the ribosome is prone to spontaneous frameshifting on mRNA slippery sequences, whereas EF-G restricts frameshifting. EF-G helps to maintain the mRNA reading frame by guiding the A-site transfer RNA during translocation du ...[more]
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