Dataset Information

Bioaugmentation failed to enhance oil bioremediation in three soil samples from three different continents.

ABSTRACT: Soil samples from Kuwait, Lebanon, Egypt and Germany were polluted with 3% crude oil. Series of samples were left unbioaugmented, others were bioaugmented with Kuwaiti desert soil with a long history of oil pollution and still others with Kuwaiti marine biofouling material. In the samples from Kuwait, Egypt, and Germany, bioaugmentation did not enhance oil removal, whereas it did in the sample from Lebanon. Taxa from the desert-soil bioaugmented batches, but none of those from the biofouling-material bioaugmented ones, succeeded in colonizing the four studied soils. The dynamics of the hydrocarbonoclastic communities during bioremediation were monitored. Those communities differed in composition, not only according to the type of soil, but also for the same soil; at various phases of bioremediation. Although each soil seemed to have its characteristic microflora, they all were similar in harboring lower and higher actinomycetes and pseudomonads in addition to many other taxa. None of the taxa prevailed through all phases of bioremediation. The most powerful isolate in oil-removal; was Rhodococcus erythropolis (Germany), and the weakest was Arthrobacter phenanthrenivorans (Lebanon). The pure hydrocarbonoclastic isolates tolerated unusually high oil concentrations, up to 30%.

SUBMITTER: Radwan SS

PROVIDER: S-EPMC6925256 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Bioaugmentation failed to enhance oil bioremediation in three soil samples from three different continents.

Radwan Samir S SS Al-Mailem Dina M DM Kansour Mayada K MK

Scientific reports 20191220 1

Soil samples from Kuwait, Lebanon, Egypt and Germany were polluted with 3% crude oil. Series of samples were left unbioaugmented, others were bioaugmented with Kuwaiti desert soil with a long history of oil pollution and still others with Kuwaiti marine biofouling material. In the samples from Kuwait, Egypt, and Germany, bioaugmentation did not enhance oil removal, whereas it did in the sample from Lebanon. Taxa from the desert-soil bioaugmented batches, but none of those from the biofouling-mat ...[more]

PMID: 31862978

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Project description:The concurrence of structurally complex petroleum-associated contaminants at relatively high concentrations, with diverse climatic conditions and textural soil characteristics, hinders conventional bioremediation processes. Recalcitrant compounds such as high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) and heavy alkanes commonly remain after standard soil bioremediation at concentrations above regulatory limits. The present study assessed the potential of native fungal bioaugmentation as a strategy to promote the bioremediation of an aged industrially polluted soil enriched with heavy hydrocarbon fractions. Microcosms assays were performed by means of biostimulation and bioaugmentation, by inoculating a defined consortium of six potentially hydrocarbonoclastic fungi belonging to the genera Penicillium, Ulocladium, Aspergillus, and Fusarium, which were isolated previously from the polluted soil. The biodegradation performance of fungal bioaugmentation was compared with soil biostimulation (water and nutrient addition) and with untreated soil as a control. Fungal bioaugmentation resulted in a higher biodegradation of total petroleum hydrocarbons (TPH) and of HMW-PAHs than with biostimulation. TPH (C14-C35) decreased by a 39.90 ± 1.99% in bioaugmented microcosms vs. a 24.17 ± 1.31% in biostimulated microcosms. As for the effect of fungal bioaugmentation on HMW-PAHs, the 5-ringed benzo(a)fluoranthene and benzo(a)pyrene were reduced by a 36% and 46%, respectively, while the 6-ringed benzoperylene decreased by a 28%, after 120 days of treatment. Biostimulated microcosm exhibited a significantly lower reduction of 5- and 6-ringed PAHs (8% and 5% respectively). Higher TPH and HMW-PAHs biodegradation levels in bioaugmented microcosms were also associated to a significant decrease in acute ecotoxicity (EC50) by Vibrio fischeri bioluminiscence inhibition assays. Molecular profiling and counting of viable hydrocarbon-degrading bacteria from soil microcosms revealed that fungal bioaugmentation promoted the growth of autochthonous active hydrocarbon-degrading bacteria. The implementation of such an approach to enhance hydrocarbon biodegradation should be considered as a novel bioremediation strategy for the treatment of the most recalcitrant and highly genotoxic hydrocarbons in aged industrially polluted soils.

Dataset Information

Bioaugmentation failed to enhance oil bioremediation in three soil samples from three different continents.

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Bioaugmentation failed to enhance oil bioremediation in three soil samples from three different continents.

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