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Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

ABSTRACT: BACKGROUND:Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in the Mashhad suburb. METHODS:From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii-specific PCR. The brain samples were investigated by T. gondii-specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers. RESULTS:In the present study, Toxoplasma-like oocysts were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T. gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis. The isolated genotyped was type II. Besides, eight Toxoplasma-positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. CONCLUSIONS:Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

SUBMITTER: Khodaverdi M

PROVIDER: S-EPMC6925478 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

Khodaverdi Majid M Razmi Gholamreza G

BMC veterinary research 20191221 1

<h4>Background</h4>Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in the Mashhad suburb.<h4>Methods</h4>From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flo ...[more]

PMID: 31864371

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Project description:BACKGROUND: Toxoplasma gondii is prevalent in most areas of the world and may cause abortions or neonatal complications in humans. As the only definitive host, cats play an important role in the epidemiology of the disease. Infection rates in cats, especially stray or free-living cats, are considered to be the best sentinels of the level of T. gondii in the environment. The T. gondii infection can be diagnosed in different ways with different methods depending on the target. However, little information on T. gondii infection in cats was available in Shanghai, China. Moreover reports on prevalence of circulating antigens, antibodies and DNA of T. gondii in the same study are rare. METHODS: In the present study, the presence of antibodies (Ab), circulating antigens (CA), and/or DNA of Toxoplasma gondii in samples from 145 stray or unwanted cats from 6 animal shelters in Shanghai (China) was determined in order to estimate the prevalence of T. gondii infection, by Ab-ELISA, CA-ELISA, and nested-PCR, respectively. RESULTS: The positive rates for the antibodies, circulating antigen and DNA of T. gondii were 11.7% (17 of 145), 5.5% (8 of 145) and 5.71% (2 of 35), respectively. No cat tested was positive by both the Ab-ELISA and the CA-ELISA, but the results of the PCR were consistent with the CA-ELISA assay. Therefore, the overall estimated prevalence of toxoplasmosis was 17.2% (25 of 145). According to our results, the positive rates of specific antibodies and circulating antigen of T. gondii were significantly different between adult cats (>1?year old) and juvenile cats (?1?year old); the former was 13.5% versus 3.9% by Ab-ELISA, while the latter was 1.7% versus 23.1% by CA-ELISA. From the results obtained with all three detection methods used in this study, the rate of infection was not significantly different between male and female cats (P ?0.05); and the overall rate was 17.9% for males versus 16.4% for females. CONCLUSIONS: The results suggest that detection of circulating antigens (CA) is necessary in surveys of T. gondii infection, especially for juvenile cats. Our investigation revealed that the prevalence of T. gondii infection in stray cats in Shanghai is high. Control programs are needed for stray cat populations in order to reduce the risk of zoonotic transmission of toxoplasmosis to other domestic animals and humans, especially females.

Project description:Toxoplasma gondii and Toxocara spp. are the most critical parasites common between humans and cats. The close association of cats with humans in urban areas persuaded us to investigate the prevalence of these parasites in stray and household cats and their possible role in the owners' infection. Herein, 132 and 33 fecal samples of stray and household cats, respectively, and 33 blood samples of their owners were collected in Tehran, Iran. The prevalence of T. gondii was determined by targeting the B1 gene in the feces of stray and household cats and the blood of cat owners. Furthermore, genotypes of T. gondii were identified based on the multilocus genotyping of BTUB, GRA6, SAG3, and APICO loci. Toxocara spp. were detected by targeting the second internal transcribed spacer (ITS-2) of the ribosomal DNA of these parasites in the cats' feces and the humans' blood. Also, Toxocara IgG was assessed in the human serum samples. The B1 gene amplification showed that 15.2% of stray cats, 18.2% of household cats, and 51.5% of cat owners were infected with T. gondii. The multilocus sequence analysis revealed the predominance of genotype I of T. gondii in stray cats and genotype II of T. gondii in household cats and cat owners. The amplifying of ITS-2 revealed a high prevalence of T. cati infection (47.0%) in stray cats, whereas no infection was found in the feces of household cats or the serum of cat owners. Likewise, Toxocara IgG was not detected in the serum of humans. The lower prevalence of T. gondii in stray/household cats than in the cat owners indicates the limited impact of close contact with infected cats in human toxoplasmosis. However, the high prevalence of T. cati infection in stray cats can cause contamination of the environment by excreting eggs that may lead to infecting humans through soil or water. Therefore, public health education in urban management planning is necessary for routine urban cat deworming programs and for training the healthcare workers to prevent, control, and treat these infections.

Dataset Information

Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

Publications

Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

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