Dataset Information

Molecular Detection and Differentiation of Theileria lestoquardi, T. ovis and T. annulata in Blood of Goats and Ticks in Kermanshah Province, Iran.

ABSTRACT: Background:This study was carried out to identify Theileria spp. infections in goats and ticksin Kermanshah Province, western Iran from May-Sep 2015. Methods:For differentiation of different Theileria spp. both blood and tick samples were examined by nested PCR-RFLP. Results:Light microscopy of blood smears revealed Theileria spp. infection in 22 (5.5%), while 68 (17%) of blood samples were positive using nested PCR. Out of 68 positive samples, 85.3% (58/68) and 11.7% (8/68) were respectively positive for Theileria ovis and T. lestoquardi. Mixed infection was detected in 3% (2/68) cases. Overall, 420 ixodid ticks belong to seven different hard ticks species were collected from goats. Rhipicephalus turanicus 112 (26.7%), R. sanguineus 95 (22.6%), R. bursa, 91(21.7%), Hyalomma anatolicum, 55(13.1%), H. excavatum 27(6.4%), H. marginatum, 22(5.3%) and Dermacentor marginatus, 18(4.2%) were the main tick species infesting goats. The PCR products obtained from ticks were subjected to the differentiation of Theileria species. Respectively, 2 and 8 pools of H. marginatum and R. turanicus salivary glands were infected with T. ovis and T. lestoquardi. In addition, T. annulata and T. lestoquardi infection weredetected in three pools of H. anatolicum. Conclusion:This is the first report of goats and collected ticks to Theileria spp infection in Iran. The results suggest that T. ovis has a higher prevalence than T. lestoquardi. It is also postulated H. marginatum, R. turanicus and H. anatolicum might play an important role in the field as a vector of Theileria spp in this area.

SUBMITTER: Rahmani-Varmale M

PROVIDER: S-EPMC6928382 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Publications

Molecular Detection and Differentiation of <i>Theileria lestoquardi</i>, <i>T. ovis</i> and <i>T. annulata</i> in Blood of Goats and Ticks in Kermanshah Province, Iran.

Rahmani-Varmale Mozhgan M Tavassoli Mousa M Esmaeilnejad Bijan B

Journal of arthropod-borne diseases 20190930 3

<h4>Background</h4>This study was carried out to identify <i>Theileria</i> spp. infections in goats and ticksin Kermanshah Province, western Iran from May-Sep 2015.<h4>Methods</h4>For differentiation of different <i>Theileria</i> spp. both blood and tick samples were examined by nested PCR-RFLP.<h4>Results</h4>Light microscopy of blood smears revealed <i>Theileria</i> spp. infection in 22 (5.5%), while 68 (17%) of blood samples were positive using nested PCR. Out of 68 positive samples, 85.3% (5 ...[more]

PMID: 31879669

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Project description:BackgroundTicks and tick-borne pathogens (TBPs) represent a significant economic burden to cattle farming in sub-Saharan Africa including Nigeria. However, in the northern part of this country, where the largest livestock population resides, little is known about the contemporary diversity of ticks and TBPs. This area is particularly vulnerable to climate change, undergoing marked transformation of habitat and associated flora and fauna that is also likely to include ticks. This study aimed to document the occurrence of tick species and Apicomplexan TBPs in cattle from north-western Nigeria.MethodsIn 2017, ticks were collected from cattle in Zamfara and Sokoto States and identified morphologically. Additionally, a subset of ticks was screened molecularly for the detection of apicomplexan DNA.ResultsA total of 494 adult ticks were collected from 80 cattle in Zamfara and 65 cattle in Sokoto State. Nine tick species were encountered, among which the presence of one, Hyalomma turanicum, had not previously been recorded in Nigeria. Hyalomma rufipes was the most prevalent tick infesting cattle in Zamfara State (76%), while Hyalomma dromedarii was the most prevalent in Sokoto State (44%), confirming the widespread transfer of this species from camels onto livestock and its adaptation to cattle in the region. Of 159 ticks screened, 2 out of 54 (3.7%) from Zamfara State and 29 out of 105 (27.6%) from Sokoto State harboured DNA of Theileria annulata, the agent of tropical theileriosis.ConclusionsThis study confirms the presence of a broad diversity of tick species in cattle from north-western Nigeria, providing the first locality records for Zamfara State. The occurrence of H. turanicum indicates a distribution of this tick beyond northern Africa. This study provides the first report for T. annulata in Nigerian ticks. Given its enormous burden on livestock farming in north Africa and across Asia, further investigations are needed to better understand its epidemiology, vector transmission and potential clinical significance in cattle from northern Nigeria and neighbouring Sahelian countries.

Dataset Information

Molecular Detection and Differentiation of Theileria lestoquardi, T. ovis and T. annulata in Blood of Goats and Ticks in Kermanshah Province, Iran.

Publications

Molecular Detection and Differentiation of <i>Theileria lestoquardi</i>, <i>T. ovis</i> and <i>T. annulata</i> in Blood of Goats and Ticks in Kermanshah Province, Iran.

Similar Datasets

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