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HOXC-AS1-MYC regulatory loop contributes to the growth and metastasis in gastric cancer.

ABSTRACT: BACKGROUND:Gastric cancer (GC) is one of the most prevalent and deadly malignancies worldwide. Accumulating reports have indicated the participation of long non-coding RNAs (lncRNAs) in the onset and progression of GC. METHODS:GSE109476 data was utilized to screen out lncRNAs dysregulated in GC. Gene expressions were determined by qRT-PCR and western blot. Both in vitro and in vivo experiments were carried out to assess the function of HOXC-AS1 in GC. The association between genes was verified via RIP, ChIP, CoIP, RNA pull down and luciferase reporter assays, as appropriate. RESULTS:HOXC-AS1 was discovered to be upregulated in GC and located both in cytoplasm and in nucleus in GC cells. Functionally, inhibition of HOXC-AS1 restrained GC cell growth and metastasis both in vitro and in vivo. Moreover, HOXC-AS1 was proved to be trans-activated by c-MYC in GC. In return, HOXC-AS1 positively regulated MYC expression in GC through targeting miR-590-3p/MYC axis in cytoplasm and modulating BRG1/?-catenin complex-activated MYC transcription in nucleus. Furthermore, the rescue assays verified that MYC mediated HOXC-AS1-affected GC progression. CONCLUSION:Our research illustrated a feedback loop of HOXC-AS1-MYC in aggravating GC cell growth and metastasis, highlighting HOXC-AS1 as a promising target for GC diagnosis and treatment.

SUBMITTER: Dong Y

PROVIDER: S-EPMC6929373 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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HOXC-AS1-MYC regulatory loop contributes to the growth and metastasis in gastric cancer.

Dong Yangyang Y Li Xinyu X Lin Zhibin Z Zou Wenbing W Liu Yan Y Qian Huiyang H Jia Jing J

Journal of experimental & clinical cancer research : CR 20191223 1

<h4>Background</h4>Gastric cancer (GC) is one of the most prevalent and deadly malignancies worldwide. Accumulating reports have indicated the participation of long non-coding RNAs (lncRNAs) in the onset and progression of GC.<h4>Methods</h4>GSE109476 data was utilized to screen out lncRNAs dysregulated in GC. Gene expressions were determined by qRT-PCR and western blot. Both in vitro and in vivo experiments were carried out to assess the function of HOXC-AS1 in GC. The association between genes ...[more]

PMID: 31870402

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HOXC-AS1-MYC regulatory loop contributes to the growth and metastasis in gastric cancer.

Publications

HOXC-AS1-MYC regulatory loop contributes to the growth and metastasis in gastric cancer.

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