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Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water.


ABSTRACT: Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly from chemically untreated biomass, to low-weight, soluble oligoxylosaccharides in >70% yields.

SUBMITTER: Ostadjoo S 

PROVIDER: S-EPMC6930478 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water.

Ostadjoo Shaghayegh S   Hammerer Fabien F   Dietrich Karolin K   Dumont Marie-Josée MJ   Friščić Tomislav T   Auclair Karine K  

Molecules (Basel, Switzerland) 20191120 23


Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from <i>Thermomyces</i> <i>lanuginosus</i> is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly f  ...[more]

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