Dataset Information

Oesophageal eosinophilia accompanies food allergy to hen egg white protein in young pigs.

ABSTRACT:

Background

Esophagitis with eosinophilia, inflammation, and fibrosis represent a chronic condition in humans with food allergies.

Objective

In this investigation, we asked whether esophagitis with an eosinophilic component is observed in young pigs rendered allergic to hen egg white protein (HEWP).

Methods

Food allergy was induced in young pigs using two protocols. In one protocol, sensitized pigs were challenged by gavage with a single dose of HEWP. Clinical signs were monitored for 24 hours, and then, gastrointestinal (GI) tissues were collected for histological examination. The phenotype of circulating, ovalbumin (OVA)-specific T cells also was examined in HEWP challenged animals. In the second protocol, sensitized animals were fed HEWP for 28 days. Animals were then examined by endoscopy and gastrointestinal tissues collected for histological examination.

Results

In pigs challenged by gavage with HEWP, clinical signs were noted in 5/6 pigs including diarrhoea, emesis, and skin rash. Clinical signs were not seen in any control group. Histological analysis revealed significant levels of oesophageal eosinophilic infiltration (P < .05) in 4/6 of these animals, with two also displaying eosinophilic infiltration in the stomach. Eosinophils were not increased in ileum or colon samples. Increased numbers of circulating, OVA-specific CD4⁺ T cells also were observed in pigs that received HEWP by gavage. In the group of animals fed HEWP, endoscopy revealed clinical signs of esophagitis including oedema, granularity, white spots, and furrowing, while histology revealed oedema, immune cell infiltration, and basal zone hyperplasia.

Conclusions and clinical relevance

Food allergy in the pig can be associated with esophagitis based on histological and endoscopic findings, including eosinophilic infiltration. The young pig may, therefore, be a useful large animal model for the study of eosinophilic esophagitis in humans.

SUBMITTER: Plundrich NJ

PROVIDER: S-EPMC6930966 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Publications

Oesophageal eosinophilia accompanies food allergy to hen egg white protein in young pigs.

Plundrich Nathalie J NJ Smith Andrew R AR Borst Luke B LB Snider Douglas B DB Käser Tobias T Dellon Evan S ES Blikslager Anthony T AT Odle Jack J Lila Mary Ann MA Laster Scott M SM

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 20191126 1

<h4>Background</h4>Esophagitis with eosinophilia, inflammation, and fibrosis represent a chronic condition in humans with food allergies.<h4>Objective</h4>In this investigation, we asked whether esophagitis with an eosinophilic component is observed in young pigs rendered allergic to hen egg white protein (HEWP).<h4>Methods</h4>Food allergy was induced in young pigs using two protocols. In one protocol, sensitized pigs were challenged by gavage with a single dose of HEWP. Clinical signs were mon ...[more]

PMID: 31702085

Similar Datasets

Project description:Chemical bonding at the active site of hen egg-white lysozyme (HEWL) is analyzed on the basis of Bader's quantum theory of atoms in molecules [QTAIM; Bader (1994), Atoms in Molecules: A Quantum Theory. Oxford University Press] applied to electron-density maps derived from a multipole model. The observation is made that the atomic displacement parameters (ADPs) of HEWL at a temperature of 100?K are larger than ADPs in crystals of small biological molecules at 298?K. This feature shows that the ADPs in the cold crystals of HEWL reflect frozen-in disorder rather than thermal vibrations of the atoms. Directly generalizing the results of multipole studies on small-molecule crystals, the important consequence for electron-density analysis of protein crystals is that multipole parameters cannot be independently varied in a meaningful way in structure refinements. Instead, a multipole model for HEWL has been developed by refinement of atomic coordinates and ADPs against the X-ray diffraction data of Wang and coworkers [Wang et al. (2007), Acta Cryst. D63, 1254-1268], while multipole parameters were fixed to the values for transferable multipole parameters from the ELMAM2 database [Domagala et al. (2012), Acta Cryst. A68, 337-351] . Static and dynamic electron densities based on this multipole model are presented. Analysis of their topological properties according to the QTAIM shows that the covalent bonds possess similar properties to the covalent bonds of small molecules. Hydrogen bonds of intermediate strength are identified for the Glu35 and Asp52 residues, which are considered to be essential parts of the active site of HEWL. Furthermore, a series of weak C-H...O hydrogen bonds are identified by means of the existence of bond critical points (BCPs) in the multipole electron density. It is proposed that these weak interactions might be important for defining the tertiary structure and activity of HEWL. The deprotonated state of Glu35 prevents a distinction between the Phillips and Koshland mechanisms.

Dataset Information

Oesophageal eosinophilia accompanies food allergy to hen egg white protein in young pigs.

Background

Objective

Methods

Results

Conclusions and clinical relevance

Publications

Oesophageal eosinophilia accompanies food allergy to hen egg white protein in young pigs.

Similar Datasets

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