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Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag.


ABSTRACT: Data in this article are associated with the research article "High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag" (Li et al., 2019) [1]. Data are provided on the development of a system for high-throughput (HTP) screening of mutants through the comparison of the activity ratios of an applicable enzyme and its mutants to a suitable tag enzyme in cell lysates of their fused forms, with Escherichia coli alkaline phosphatase (ECAP) as the tag fused to the N-terminus of Pseudomonas Aeruginosa arylsulfatase (PAAS) and its mutants via a flexible linker. Data were made publicly available for further analyses.

SUBMITTER: Li Y 

PROVIDER: S-EPMC6931123 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag.

Li Yaping Y   Chong Huimin H   Zhang Xiang X   Yang Xiaolan X   Liao Fei F  

Data in brief 20191213


Data in this article are associated with the research article "High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag" (Li et al., 2019) [1]. Data are provided on the development of a system for high-throughput (HTP) screening of mutants through the comparison of the activity ratios of an applicable enzyme and its mutants to a suitable tag enzyme in cell lysates of their fused forms, with <i>Escherichia coli</i> alkaline phosphatase (ECAP) as the tag  ...[more]

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