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Nickel induces inflammatory activation via NF-?B, MAPKs, IRF3 and NLRP3 inflammasome signaling pathways in macrophages.


ABSTRACT: Nickel (Ni), an environmental hazard, widely causes allergic contact hypersensitivity worldwide. Despite that Ni-stimulated pro-inflammatory response is vital in allergy, the underlying molecular mechanisms remain largely unclear. Here, we demonstrated that NiCl2 activated nuclear factor kappa B (NF-?B), mitogen-activated protein kinases (MAPKs) and interferon regulatory factor 3 (IRF3) signaling pathways in primary bone marrow-derived macrophages (BMDMs), leading to the altered transcription levels of interleukin-1? (IL-1?), -6, -8, -18, tumor necrosis factor-? (TNF-?) and interferon ? (INF-?). We also found that nickel chloride (NiCl2) activated Nod-like receptor 3 (NLRP3) inflammasome pathway, resulting in the proteolytic cleavage and release of IL-1?. NiCl2 induced the accumulation of mitochondrial reactive oxygen species (mtROS) and the release of mitochondrial DNA (mtDNA), thus activating NLRP3 inflammasome pathway. Additionally, NiCl2-induced apoptosis was dependent on the generation of mtROS, and caspase-1 activation might also partly contribute to the apoptotic process. Altogether, abovementioned results indicate that NiCl2 induces inflammatory activation in BMDMs via NF-?B, MAPKs, IRF3 signaling pathways as well as NLRP3 inflammasome pathway, which provides a mechanism to improve the efficiency of treatment against Ni-induced allergic reactions.

SUBMITTER: Guo H 

PROVIDER: S-EPMC6932914 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Nickel induces inflammatory activation via NF-κB, MAPKs, IRF3 and NLRP3 inflammasome signaling pathways in macrophages.

Guo Hongrui H   Liu Huan H   Jian Zhijie Z   Cui Hengmin H   Fang Jing J   Zuo Zhicai Z   Deng Junliang J   Li Yinglun Y   Wang Xun X   Zhao Ling L   Geng Yi Y   Ouyang Ping P   Lai Weiming W   Chen Zhengli Z   Huang Chao C  

Aging 20191210 23


Nickel (Ni), an environmental hazard, widely causes allergic contact hypersensitivity worldwide. Despite that Ni-stimulated pro-inflammatory response is vital in allergy, the underlying molecular mechanisms remain largely unclear. Here, we demonstrated that NiCl<sub>2</sub> activated nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPKs) and interferon regulatory factor 3 (IRF3) signaling pathways in primary bone marrow-derived macrophages (BMDMs), leading to the altered trans  ...[more]

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