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IL-10 induces MC3T3-E1 cells differentiation towards osteoblastic fate in murine model.

ABSTRACT: Interleukin-10 (IL-10) displays well-documented anti-inflammatory effects, but its effects on osteoblast differentiation have not been investigated. In this study, we found IL-10 negatively regulates microRNA-7025-5p (miR-7025-5p), the down-regulation of which enhances osteoblast differentiation. Furthermore, through luciferase reporter assays, we found evidence that insulin-like growth factor 1 receptor (IGF1R) is a miR-7025-5p target gene that positively regulates osteoblast differentiation. In vivo studies indicated that the pre-injection of IL-10 leads to increased bone formation, while agomiR-7025-5p injection delays fracture healing. Taken together, these results indicate that IL-10 induces osteoblast differentiation via regulation of the miR-7025-5p/IGF1R axis. IL-10 therefore represents a promising therapeutic strategy to promote fracture healing.

SUBMITTER: Xiong Y

PROVIDER: S-EPMC6933380 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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IL-10 induces MC3T3-E1 cells differentiation towards osteoblastic fate in murine model.

Xiong Yuan Y Yan Chenchen C Chen Lang L Endo Yori Y Sun Yun Y Zhou Wu W Hu Yiqiang Y Hu Liangcong L Chen Dong D Xue Hang H Mi Bobin B Liu Guohui G

Journal of cellular and molecular medicine 20191121 1

Interleukin-10 (IL-10) displays well-documented anti-inflammatory effects, but its effects on osteoblast differentiation have not been investigated. In this study, we found IL-10 negatively regulates microRNA-7025-5p (miR-7025-5p), the down-regulation of which enhances osteoblast differentiation. Furthermore, through luciferase reporter assays, we found evidence that insulin-like growth factor 1 receptor (IGF1R) is a miR-7025-5p target gene that positively regulates osteoblast differentiation. I ...[more]

PMID: 31755174

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