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Methylations in complex carbapenem biosynthesis are catalyzed by a single cobalamin-dependent radical S-adenosylmethionine enzyme.


ABSTRACT: Complex carbapenem ?-lactam antibiotics contain diverse C6 alkyl substituents constructed by cobalamin-dependent radical SAM enzymes. TokK installs the C6 isopropyl chain found in asparenomycin. Time-course analyses of TokK and its ortholog ThnK, which forms the C6 ethyl chain of thienamycin, indicate that catalysis occurs through a sequence of discrete, non-processive methyl transfers.

SUBMITTER: Sinner EK 

PROVIDER: S-EPMC6934041 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Methylations in complex carbapenem biosynthesis are catalyzed by a single cobalamin-dependent radical S-adenosylmethionine enzyme.

Sinner Erica K EK   Lichstrahl Michael S MS   Li Rongfeng R   Marous Daniel R DR   Townsend Craig A CA  

Chemical communications (Cambridge, England) 20191201 99


Complex carbapenem β-lactam antibiotics contain diverse C6 alkyl substituents constructed by cobalamin-dependent radical SAM enzymes. TokK installs the C6 isopropyl chain found in asparenomycin. Time-course analyses of TokK and its ortholog ThnK, which forms the C6 ethyl chain of thienamycin, indicate that catalysis occurs through a sequence of discrete, non-processive methyl transfers. ...[more]

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