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Spatio-temporal expression and distribution of collagen VI during zebrafish development.


ABSTRACT: Collagen VI (ColVI) is an extracellular matrix (ECM) protein involved in a range of physiological and pathological conditions. Zebrafish (Danio rerio) is a powerful model organism for studying vertebrate development and for in vivo analysis of tissue patterning. Here, we performed a thorough characterization of ColVI gene and protein expression in zebrafish during development and adult life. Bioinformatics analyses confirmed that zebrafish genome contains single genes encoding for ?1(VI), ?2(VI) and ?3(VI) ColVI chains and duplicated genes encoding for ?4(VI) chains. At 1 day post-fertilization (dpf) ColVI transcripts are expressed in myotomes, pectoral fin buds and developing epidermis, while from 2 dpf abundant transcript levels are present in myosepta, pectoral fins, axial vasculature, gut and craniofacial cartilage elements. Using newly generated polyclonal antibodies against zebrafish ?1(VI) protein, we found that ColVI deposition in adult fish delineates distinct domains in the ECM of several organs, including cartilage, eye, skin, spleen and skeletal muscle. Altogether, these data provide the first detailed characterization of ColVI expression and ECM deposition in zebrafish, thus paving the way for further functional studies in this species.

SUBMITTER: Tonelotto V 

PROVIDER: S-EPMC6934817 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Spatio-temporal expression and distribution of collagen VI during zebrafish development.

Tonelotto Valentina V   Trapani Valeria V   Bretaud Sandrine S   Heumüller Stefanie Elisabeth SE   Wagener Raimund R   Ruggiero Florence F   Bonaldo Paolo P  

Scientific reports 20191227 1


Collagen VI (ColVI) is an extracellular matrix (ECM) protein involved in a range of physiological and pathological conditions. Zebrafish (Danio rerio) is a powerful model organism for studying vertebrate development and for in vivo analysis of tissue patterning. Here, we performed a thorough characterization of ColVI gene and protein expression in zebrafish during development and adult life. Bioinformatics analyses confirmed that zebrafish genome contains single genes encoding for α1(VI), α2(VI)  ...[more]

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