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Efficacy of Active Immunization With Attenuated ?-Hemolysin and Panton-Valentine Leukocidin in a Rabbit Model of Staphylococcus aureus Necrotizing Pneumonia.


ABSTRACT: Staphylococcus aureus is a common pathogen causing infections in humans with various degrees of severity, with pneumonia being one of the most severe infections. In as much as staphylococcal pneumonia is a disease driven in large part by ?-hemolysin (Hla) and Panton-Valentine leukocidin (PVL), we evaluated whether active immunization with attenuated forms of Hla (HlaH35L/H48L) alone, PVL components (LukS-PVT28F/K97A/S209A and LukF-PVK102A) alone, or combination of all 3 toxoids could prevent lethal challenge in a rabbit model of necrotizing pneumonia caused by the USA300 community-associated methicillin-resistant S. aureus (MRSA). Rabbits vaccinated with Hla toxoid alone or PVL components alone were only partially protected against lethal pneumonia, whereas those vaccinated with all 3 toxoids had 100% protection against lethality. Vaccine-mediated protection correlated with induction of polyclonal antibody response that neutralized not only ?-hemolysin and PVL, but also other related toxins, produced by USA300 and other epidemic MRSA clones.

SUBMITTER: Tran VG 

PROVIDER: S-EPMC6936001 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Efficacy of Active Immunization With Attenuated α-Hemolysin and Panton-Valentine Leukocidin in a Rabbit Model of Staphylococcus aureus Necrotizing Pneumonia.

Tran Vuvi G VG   Venkatasubramaniam Arundhathi A   Adhikari Rajan P RP   Krishnan Subramaniam S   Wang Xing X   Le Vien T M VTM   Le Hoan N HN   Vu Trang T T TTT   Schneider-Smith Erika E   Aman M Javad MJ   Diep Binh An BA  

The Journal of infectious diseases 20200101 2


Staphylococcus aureus is a common pathogen causing infections in humans with various degrees of severity, with pneumonia being one of the most severe infections. In as much as staphylococcal pneumonia is a disease driven in large part by α-hemolysin (Hla) and Panton-Valentine leukocidin (PVL), we evaluated whether active immunization with attenuated forms of Hla (HlaH35L/H48L) alone, PVL components (LukS-PVT28F/K97A/S209A and LukF-PVK102A) alone, or combination of all 3 toxoids could prevent let  ...[more]

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