Ontology highlight
ABSTRACT:
SUBMITTER: van Es JH
PROVIDER: S-EPMC6936398 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20191216 52
Cycling intestinal Lgr5<sup>+</sup> stem cells are intermingled with their terminally differentiated Paneth cell daughters at crypt bottoms. Paneth cells provide multiple secreted (e.g., Wnt, EGF) as well as surface-bound (Notch ligand) niche signals. Here we show that ablation of Paneth cells in mice, using a diphtheria toxin receptor gene inserted into the P-lysozyme locus, does not affect the maintenance of Lgr5<sup>+</sup> stem cells. Flow cytometry, single-cell sequencing, and histological ...[more]