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Enteroendocrine and tuft cells support Lgr5 stem cells on Paneth cell depletion.


ABSTRACT: Cycling intestinal Lgr5+ stem cells are intermingled with their terminally differentiated Paneth cell daughters at crypt bottoms. Paneth cells provide multiple secreted (e.g., Wnt, EGF) as well as surface-bound (Notch ligand) niche signals. Here we show that ablation of Paneth cells in mice, using a diphtheria toxin receptor gene inserted into the P-lysozyme locus, does not affect the maintenance of Lgr5+ stem cells. Flow cytometry, single-cell sequencing, and histological analysis showed that the ablated Paneth cells are replaced by enteroendocrine and tuft cells. As these cells physically occupy Paneth cell positions between Lgr5 stem cells, they serve as an alternative source of Notch signals, which are essential for Lgr5+ stem cell maintenance. Our combined in vivo results underscore the adaptive flexibility of the intestine in maintaining normal tissue homeostasis.

SUBMITTER: van Es JH 

PROVIDER: S-EPMC6936398 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Enteroendocrine and tuft cells support Lgr5 stem cells on Paneth cell depletion.

van Es Johan H JH   Wiebrands Kay K   López-Iglesias Carmen C   van de Wetering Marc M   Zeinstra Laura L   van den Born Maaike M   Korving Jeroen J   Sasaki Nobuo N   Peters Peter J PJ   van Oudenaarden Alexander A   Clevers Hans H  

Proceedings of the National Academy of Sciences of the United States of America 20191216 52


Cycling intestinal Lgr5<sup>+</sup> stem cells are intermingled with their terminally differentiated Paneth cell daughters at crypt bottoms. Paneth cells provide multiple secreted (e.g., Wnt, EGF) as well as surface-bound (Notch ligand) niche signals. Here we show that ablation of Paneth cells in mice, using a diphtheria toxin receptor gene inserted into the P-lysozyme locus, does not affect the maintenance of Lgr5<sup>+</sup> stem cells. Flow cytometry, single-cell sequencing, and histological  ...[more]

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