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Larval Rearing Methods for Small-scale Production of Healthy Zebrafish.

ABSTRACT: Raising larvae of Danio rerio Hamilton (zebrafish) is a challenging task that requires skill and a significant daily time investment. We have developed a simple nursery and a husbandry regimen that streamlines procedures and is feasible for small laboratories to carry out in the absence of support staff. The nursery is inexpensive to build and easy to maintain. The regimen uses a simple benchtop nursery that houses up to 300 larvae. Feeding is simplified by using defined volumes of microencapsulated feeds with only 1 type of live prey as a dietary supplement, Artemia franciscana Leach (brine shrimp). Tests of the regimen using wild-type lines showed that it supports timely entry into the metamorphic period and supports survival rates of at least 75%. Further, inexperienced users were able to raise larvae successfully. Here, we describe how to assemble the nursery and how to carry out the feeding and care regimen.

SUBMITTER: Norton A

PROVIDER: S-EPMC6936768 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Larval Rearing Methods for Small-scale Production of Healthy Zebrafish.

Norton Alena A Franse Kitt F KF Daw Tierney T Gordon Latanya L Vitiello Peter F PF Kinkel Mary D MD

Eastern biologist 20190101 Spec Issue

Raising larvae of <i>Danio rerio</i> Hamilton (zebrafish) is a challenging task that requires skill and a significant daily time investment. We have developed a simple nursery and a husbandry regimen that streamlines procedures and is feasible for small laboratories to carry out in the absence of support staff. The nursery is inexpensive to build and easy to maintain. The regimen uses a simple benchtop nursery that houses up to 300 larvae. Feeding is simplified by using defined volumes of microe ...[more]

PMID: 31890349

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Project description:BACKGROUND:Standardized larval rearing units for mosquito production are essential for the establishment of a mass-rearing facility. Two larval rearing units, developed respectively by the Guangzhou Wolbaki Biotech Co. Ltd. (Wolbaki) and Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture (FAO/IAEA-IPCL), are tested to assess their potential uses to mass-rear the larval stages of Aedes albopictus in support of the establishment of a medium-scale mosquito facility for the application of mosquito genetic control strategies. METHODS:The triple Wolbachia-infected Ae. albopictus strain (HC strain) was used in this study. The effects of larval densities of two larval rearing trays (corresponding to 2.4, 3.0 and 3.6 larvae/cm2) and tray size/position (top, middle and bottom layers) on the pupae production and larval survival were assessed when trays were stacked within the larval rearing units. The male pupae production, female pupae contamination after sex separation, and male mating competitiveness were also studied by using both larval rearing units in their entirety. RESULTS:The optimal larval rearing density for Wolbaki-tray (Wol-tray) was 6,600 larvae (equal to 3.0 larvae/cm2) and 18,000 larvae (3.6 larvae/cm2) for the FAO/IAEA-IPCL tray (IAEA-tray). No significant difference in pupae production was observed when trays were stacked within top, middle or bottom layers for both units. At thirty-four hours after the first pupation, the average male pupae production was (0.89 × 105) for the Wol-unit and (3.16 × 105) for the IAEA-unit. No significant difference was observed in female pupae contamination between these two units. The HC males showed equal male mating competitiveness to wild type males for mating with wild type females in large cages, regardless of whether they were reared in the Wol-unit or IAEA-unit. CONCLUSIONS:The current study has indicated that both the Wol-unit and IAEA-unit are suitable for larvae mass-rearing for Ae. albopictus. However, the IAEA-unit, with higher male production and less space required compared to the Wol-unit, is recommended to be used in support of the establishment of a medium-sized mosquito facility.

Dataset Information

Larval Rearing Methods for Small-scale Production of Healthy Zebrafish.

Publications

Larval Rearing Methods for Small-scale Production of Healthy Zebrafish.

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