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Intracellular Reassociation of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells.

ABSTRACT: Human immunodeficiency virus Type 1 (HIV-1) is the major cause of acquired immune deficiency syndrome (AIDS). In 2014, it was estimated that 1.2 million people died from AIDS-related illnesses. RNA interference-based therapy to block HIV replication is a field that, as of now, is without any FDA-approved drugs available for clinical use. In this chapter we describe a protocol for testing and utilizing a new approach that relies on reassociation of RNA-DNA hybrids activating RNAi and blocking HIV replication in human cells.

SUBMITTER: Martins AN

PROVIDER: S-EPMC6941940 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Intracellular Reassociation of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells.

Martins Angelica N AN Ke Weina W Jawahar Vaishnavi V Striplin Morriah M Striplin Caryn C Freed Eric O EO Afonin Kirill A KA

Methods in molecular biology (Clifton, N.J.) 20170101

Human immunodeficiency virus Type 1 (HIV-1) is the major cause of acquired immune deficiency syndrome (AIDS). In 2014, it was estimated that 1.2 million people died from AIDS-related illnesses. RNA interference-based therapy to block HIV replication is a field that, as of now, is without any FDA-approved drugs available for clinical use. In this chapter we describe a protocol for testing and utilizing a new approach that relies on reassociation of RNA-DNA hybrids activating RNAi and blocking HIV ...[more]

PMID: 28730446

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