Preparation and Characterisation of Polyphenol-HP-?-Cyclodextrin Inclusion Complex that Protects Lamb Tripe Protein against Oxidation.
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ABSTRACT: Grape seed extract (GSE) displays strong antioxidant activity, but its instability creates barriers to its applications. Herein, three HP-?-CD/GSE inclusion complexes with host-guest ratios of 1:0.5, 1:1, and 1:2 were successfully prepared by co-precipitation method to improve stability. Successful embedding of GSE in the HP-?-CD cavity was confirmed by fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM) analyses. The Autodock Tools 1.5.6 was used to simulate the three-dimensional supramolecular structure of the inclusion complex of 2-hydroxypropyl-?-cyclodextrin and grape seed extract (HP-?-CD/GSE) by molecular docking. The MALDI-TOF-MS technology and chemical database Pubchem, and structural database PDB were combined to reconstitute the three-dimensional structure of target protein. The binding mode of the HP-?-CD/GSE inclusion complex to target protein was studied at the molecular level, and the antioxidant ability of the resulting HP-?-CD/GSE inclusion complexes was investigated by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The effects of HP-?-CD/GSE on myofibrillar protein from lamb tripe were also investigated under oxidative conditions. The positions and interactions of the binding sites of HP-?-CD/GSE inclusion complexes and target protein receptors were simulated by molecular docking. The results showed that HP-?-CD/GSE inclusion complexes were successfully prepared, optimally at a molar ratio of 1:2. At low (5 ?mol/g) to medium (105 ?mol/g) concentrations, HP-?-CD/GSE inclusion complexes decreased the carbonyl content, hydrophobicity, and protein aggregation of myofibrillar protein from lamb tripe, and increased the sulphydryl content. Furthermore, high concentration (155 ?mol/g) of HP-?-CD/GSE inclusion complexes promoted protein oxidation.
SUBMITTER: Li W
PROVIDER: S-EPMC6943433 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
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