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Vitamin D Inhibition of TRPV5 Expression During Osteoclast Differentiation.

ABSTRACT: Background:Vitamin D is an important steroid that can regulate bone metabolism including osteoclast (OC) differentiation. Transient receptor potential cation channel subfamily V member 5 (TRPV5), is a calcium channel protein involved in OC differentiation. However, the impact of vitamin D on TRPV5 expression during OC differentiation is not clear. Objectives:To determine if 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) regulates the expression of TRPV5 during OC differentiation. Methods:Bone marrow mononuclear macrophage (BMMs) were induced to differentiate into OC with or without treatment with 10 nM 1,25(OH)2D3. The expression levels of vitamin D receptor (VDR) and TRPV5 were examined. The expression of several OC markers, including tartrate resistant acid phosphatase (TRAP), carbonic anhydrase II (Ca II), cathepsin K (CTSK), and vacuolar-type H+-ATPase (V-ATPase) were also detected. Results:We found that the VDR was expressed in murine bone marrow-derived macrophages at the early stage of OC differentiation. TRPV5 expression was increased during OC differentiation, which was down-regulated by 1,25(OH)2D3 after a prolonged exposure. The 1,25(OH)2D3 and TRPV5 inhibitors inhibited OC differentiation. Conclusions:1,25(OH)2D3 can inhibit TRPV5 expression as well as TRPV5 inhibitors during OC differentiation. This suggests that 1,25(OH)2D3 may suppress OC differentiation by inhibiting TRPV5 expression.

SUBMITTER: Gu J

PROVIDER: S-EPMC6948119 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Vitamin D Inhibition of TRPV5 Expression During Osteoclast Differentiation.

Gu Jianhong J Tong Xishuai X Chen Yang Y Zhang Chuang C Ma Tianhong T Li Saihui S Min Wenyan W Yuan Yan Y Liu Xuezhong X Bian Jianchun J Liu Zongping Z

International journal of endocrinology and metabolism 20191014 4

<h4>Background</h4>Vitamin D is an important steroid that can regulate bone metabolism including osteoclast (OC) differentiation. Transient receptor potential cation channel subfamily V member 5 (TRPV5), is a calcium channel protein involved in OC differentiation. However, the impact of vitamin D on TRPV5 expression during OC differentiation is not clear.<h4>Objectives</h4>To determine if 1,25-dihydroxyvitamin D3 (1,25(OH)<sub>2</sub>D<sub>3</sub>) regulates the expression of TRPV5 during OC dif ...[more]

PMID: 31998380

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Dataset Information

Vitamin D Inhibition of TRPV5 Expression During Osteoclast Differentiation.

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Vitamin D Inhibition of TRPV5 Expression During Osteoclast Differentiation.

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