Ontology highlight
ABSTRACT:
SUBMITTER: Allen F
PROVIDER: S-EPMC6949135 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Allen Felicity F Crepaldi Luca L Alsinet Clara C Strong Alexander J AJ Kleshchevnikov Vitalii V De Angeli Pietro P Páleníková Petra P Khodak Anton A Kiselev Vladimir V Kosicki Michael M Bassett Andrew R AR Harding Heather H Galanty Yaron Y Muñoz-Martínez Francisco F Metzakopian Emmanouil E Jackson Stephen P SP Parts Leopold L
Nature biotechnology 20181127
The DNA mutation produced by cellular repair of a CRISPR-Cas9-generated double-strand break determines its phenotypic effect. It is known that the mutational outcomes are not random, but depend on DNA sequence at the targeted location. Here we systematically study the influence of flanking DNA sequence on repair outcome by measuring the edits generated by >40,000 guide RNAs (gRNAs) in synthetic constructs. We performed the experiments in a range of genetic backgrounds and using alternative CRISP ...[more]