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Evaluation of pre-treatment methods for Lantana camara stem for enhanced enzymatic saccharification.

ABSTRACT: This study evaluates certain pre-treatment methods for Lantana camara stem for efficient conversion to fermentable sugars. The composition analysis of L. camara stem showed 66.8% (w/w) holocellulose, 34.9% (w/w) cellulose and 17% (w/w) hemicellulose. Comparative analysis of various chemical, physical or physico-chemical pre-treatments on L. camara stem was performed. Of all pretreatment methods used, pre-treatment with 1% (v/v) H₂SO₄ assisted autoclaving gave maximum total reducing sugar yield 132.7 mg/g (13.2 g/L) of raw biomass in pretreated hydrolysate. Major contribution to total reducing sugar was from hemicellulosic fraction, because total pentose sugar yield was 119.4 mg/g of raw biomass whereas, glucose released was only 10 mg/g of untreated biomass. The enzymatic saccharification of pre-treated L. camara stem by 1% (v/v) H₂SO₄ assisted autoclaving was performed with partially purified carboxymethylcellulase from Bacillus amyloliquefaciens SS35. Enzymatic saccharification at 30 °C for 48 h gave total reducing sugar yield, 63.3 mg/g of pre-treated biomass in the hydrolysate, while untreated biomass gave 43.3 mg/g of untreated biomass. The total sugar yield i.e. the sum of pre-treated biomass hydrolysate total reducing sugar (132.7 mg/g of raw biomass) and enzymatic hydrolysate total reducing sugar (63.3 mg/g of pre-treated biomass) was 196.0 mg/g of raw biomass, indicating the effectiveness of pre-treatment method. Field emission scanning electron microscopy, Fourier transform infrared and X-ray diffraction analyses displayed enhanced porosity, removal of non-cellulosic sugars and increased cellulose crystallinity, respectively, in pre-treated L. camara stem, showing the effectiveness of acid assisted autoclaving pre-treatment.

SUBMITTER: Kumar A

PROVIDER: S-EPMC6949356 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Evaluation of pre-treatment methods for Lantana camara stem for enhanced enzymatic saccharification.

Kumar Ajit A Singh Shweta S Rajulapati Vikky V Goyal Arun A

3 Biotech 20200108 2

This study evaluates certain pre-treatment methods for Lantana camara stem for efficient conversion to fermentable sugars. The composition analysis of L. camara stem showed 66.8% (w/w) holocellulose, 34.9% (w/w) cellulose and 17% (w/w) hemicellulose. Comparative analysis of various chemical, physical or physico-chemical pre-treatments on L. camara stem was performed. Of all pretreatment methods used, pre-treatment with 1% (v/v) H2SO4 assisted autoclavin ...[more]

PMID: 31988831

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Project description:BACKGROUND:Uvaria chamae (Annonaceae), Phyllantus amarus (Phyllantaceae) and Lantana camara (Verbenaceae) are empirically alleged to be used as Beninese medicinal plants in the treatment of salmonellosis. This study aimed to produce scientific data on in vitro and in vivo efficacy of Uvaria chamae, Lantana camara and Phyllantus amarus on multiresistant Salmonella spp isolated in Benin. RESULTS:After performing in vitro tests on aqueous and ethanolic extracts of these plants, only the aqueous extract of Uvaria chamae (leaves) showed the best anti-Salmonella's activity and was used for this in vivo experiment. The induction of salmonellosis revealed 9?×?108?CFU/ml was the optimal concentration triggering and maintaining symptoms in chicks. This infective concentration was used for in vivo assessment. Twenty-four hours post inoculation, the symptoms of salmonellosis (wet cloaca, diarrhea stools and somnolence) were observed in infected groups. After 7 days of treatment, the reduction of bacterial load at 100?mg/L, 200?mg/L, 400?mg/L of the extract was respectively 85%, 52.38% and 98% for Uvaria chamae, Phyllantus amarus and Lantana camara in the chick's groups infected with Salmonella Typhimurium ATCC 14028. On the other hand, colistin completely cancelled the bacterial load (reduction rate of 100%). With the groups infected with Salmonella spp (virulent strain), the reduction rate of bacterial load at 100?mg/L, 200?mg/L, 400?mg/L of extract was 0%, 98.66%, and 99.33%. The extracts at 200 and 400?mg/L were more active than colistin, which reduced the bacterial load by 33.33%. The toxicity tests did not show any negative effect of Colistin and the Uvaria chamae's extract on the biochemical and hematological parameters of the chicks. CONCLUSION:The aqueous extract of Uvaria chamae is active in vitro and in vivo on multiresistant strains of Salmonella enterica. This plant is a good candidate for the development of an improved traditional medicine for the management of salmonellosis.

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Evaluation of pre-treatment methods for Lantana camara stem for enhanced enzymatic saccharification.

Publications

Evaluation of pre-treatment methods for <i>Lantana camara</i> stem for enhanced enzymatic saccharification.

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