Unknown

Dataset Information

0

The Coumarin Derivative 5'-Hydroxy Auraptene Suppresses Osteoclast Differentiation via Inhibiting MAPK and c-Fos/NFATc1 Pathways.


ABSTRACT: The phytochemical substances, coumarin derivatives, have demonstrated antiresorptive bone effects by suppressing osteoclast differentiation in vitro and in vivo. Recently, we have identified 5'-hydroxy auraptene (5'-HA), a coumarin derivative isolated from Lotus lalambensis Schweinf, as a novel stimulator for osteoblast differentiation. In this study, we investigated the effect of 5'-HA on osteoclast differentiation of mouse bone marrow (BM) cells. The effect of 5'-HA on BM cell proliferation and osteoclast differentiation was determined by measuring cell viability and tartrate-resistant acid phosphatase (TRAP) enzyme activity, quantification of TRAP+ multinucleated cells (TRAP+MNCs), and quantitative real-time PCR (qPCR) of osteoclastic gene expression. Regulation of NF-?B, c-Fos/NFATc1, and MAPK signaling pathways by 5'-HA during osteoclastogenesis was measured by the NF-?B reporter assay and Western blot analysis. 5'-HA significantly suppresses the receptor activator of NF-?B ligand (RANKL) induced osteoclast differentiation of BM cells in a dose-dependent manner. Consistently, treatment of BM cells with 5'-HA significantly inhibited RANKL-induced activation of NF-?B and c-Fos/NFATc1 pathways in a dose-dependent manner. Furthermore, RANKL-induced phosphorylation of ERK1/2, p-38, and JNK was significantly inhibited by 5'-HA in BM cells. In conclusion, we identified 5'-HA as a novel coumarin derivative that suppresses RANKL-induced osteoclastogenesis via inhibiting c-Fos/NFATc1 and MAPK signaling pathways.

SUBMITTER: Abdallah BM 

PROVIDER: S-EPMC6949687 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

altmetric image

Publications

The Coumarin Derivative 5'-Hydroxy Auraptene Suppresses Osteoclast Differentiation via Inhibiting MAPK and c-Fos/NFATc1 Pathways.

Abdallah Basem M BM   Ali Enas M EM   Elsawy Hany H   Badr Gehan M GM   Abdel-Moneim Ashraf M AM   Alzahrani Abdullah M AM  

BioMed research international 20191228


The phytochemical substances, coumarin derivatives, have demonstrated antiresorptive bone effects by suppressing osteoclast differentiation <i>in vitro</i> and <i>in vivo</i>. Recently, we have identified 5'-hydroxy auraptene (5'-HA), a coumarin derivative isolated from <i>Lotus lalambensis</i> Schweinf, as a novel stimulator for osteoblast differentiation. In this study, we investigated the effect of 5'-HA on osteoclast differentiation of mouse bone marrow (BM) cells. The effect of 5'-HA on BM  ...[more]

Similar Datasets

| S-EPMC8801808 | biostudies-literature
| S-EPMC6167898 | biostudies-literature
| S-EPMC7358641 | biostudies-literature
| S-EPMC9571677 | biostudies-literature
| S-EPMC4245549 | biostudies-literature
| S-EPMC8353383 | biostudies-literature
| S-EPMC6688344 | biostudies-literature
| S-EPMC10460717 | biostudies-literature
| S-EPMC10720268 | biostudies-literature
| S-EPMC5771597 | biostudies-literature