Dataset Information

Detection of serum long non-coding RNA UCA1 and circular RNAs for the diagnosis of bladder cancer and prediction of recurrence.

ABSTRACT: Accumulating evidence indicates that long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) may be biomarkers for the diagnosis and prediction of recurrence in tumor patients because they play an important role in tumorigenesis and progression. In this study, the expression of lncRNA urothelial carcinoma-associated 1 (UCA1) and associated circFARSA, circSHKBP1, and circBANP was investigated in serum specimens from bladder cancer (BC) patients and healthy controls using real-time PCR. When comparing patients with BC to healthy controls, the expression of lncRNA UCA1, circFARSA, and circSHKBP1 was significantly increased. The area under the curve (AUC) of the lncRNA UCA1 and circSHKBP1 signature to distinguish BC patients from controls was 0.804. The diagnostic performance of this signature was more optimal for low volume tumors (AUC = 0.870). Moreover, we determined that the expression of circFARSA, circSHKBP1, and circBANP was higher for recurrent BC than for patients without recurrence. Receiver operating characteristic (ROC) analysis revealed that a combination of circFARSA and circBANP levels was able to discriminate the patients with tumor recurrence from those without, with an area under the ROC curve of 0.737. In conclusion, our results identified an lncRNA UCA1: circSHKBP1 panel and a circFARSA: circBANP panel for BC diagnosis and prognosis, respectively.

SUBMITTER: Pan J

PROVIDER: S-EPMC6949697 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Detection of serum long non-coding RNA UCA1 and circular RNAs for the diagnosis of bladder cancer and prediction of recurrence.

Pan Jingjing J Xie Xiaojuan X Li Huijin H Li Zhengkun Z Ren Chunfeng C Ming Liang L

International journal of clinical and experimental pathology 20190801 8

Accumulating evidence indicates that long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) may be biomarkers for the diagnosis and prediction of recurrence in tumor patients because they play an important role in tumorigenesis and progression. In this study, the expression of lncRNA urothelial carcinoma-associated 1 (UCA1) and associated circFARSA, circSHKBP1, and circBANP was investigated in serum specimens from bladder cancer (BC) patients and healthy controls using real-time PCR. When co ...[more]

PMID: 31934131

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Project description:BackgroundNrf2 (nuclear factor, erythroid 2 like 2) is believed to play a major role in neurodegenerative diseases. The present study attempts to investigate the hippocampal circRNA and lncRNA expression profiles associated with Nrf2-mediated neuroprotection.MethodsThe hippocampal mRNA, circRNA and lncRNA expression profiles of Nrf2 (-/-) mice were determined by a microarray analysis. Bioinformatics analyses, including identification of differentially expressed mRNAs (DEmRNAs), circRNAs (DEcircRNAs) and lncRNAs (DElncRNAs), DEcircRNA-miRNA-DEmRNA interaction network construction, DElncRNA-DEmRNA co-expression network construction, and biological function annotation, were conducted. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the dysregulated expression of circRNAs and lncRNAs derived from the microarray data of the hippocampus of Nrf2 (-/-) mice.ResultsCompared to wild-type Nrf2 (+/+) mice, 412 DEmRNAs (109 up- and 303 down-regulated mRNAs), 1279 DEcircRNAs (632 up- and 647 down-regulated circRNAs), and 303 DElncRNAs (50 up- and 253 down-regulated lncRNAs) were identified in the hippocampus of Nrf2 (-/-) mice. Additionally, in the qRT-PCR validation results, the expression patterns of selected DEcircRNAs and DElncRNAs were generally consistent with results in the microarray data. The DEcircRNA-miRNA-DEmRNA interaction networks revealed that mmu_circRNA_44531, mmu_circRNA_34132, mmu_circRNA_000903, mmu_circRNA_018676, mmu_circRNA_45901, mmu_circRNA_33836, mmu_circRNA_ 34137, mmu_circRNA_34106, mmu_circRNA_008691, and mmu_circRNA_003237 were predicted to compete with 47, 54, 45, 57, 63, 81, 121, 85, 181, and 43 DEmRNAs, respectively. ENSMUST00000125413, NR_028123, uc008nfy.1, AK076764, AK142725, AK080547, and AK035903 were co-expressed with 178, 89, 149, 179, 142, 55, and 112 DEmRNAs in the Nrf2 (-/-) hippocampus, respectively.ConclusionOur study might contribute to exploring the key circRNAs and lncRNAs associated with Nrf2-mediated neuroprotection.

Project description:BackgroundTo overcome the hostile hypoxic microenvironment of solid tumors, tumor cells secrete a large number of non-coding RNA-containing exosomes that facilitate tumor development and metastasis. However, the precise mechanisms of tumor cell-derived exosomes during hypoxia are unknown. Here, we aim to clarify whether hypoxia affects tumor growth and progression by transferring long non-coding RNA-urothelial cancer-associated 1 (lncRNA-UCA1) enriched exosomes secreted from bladder cancer cells.MethodsWe used bladder cancer 5637 cells with high expression of lncRNA-UCA1 as exosome-generating cells and bladder cancer UMUC2 cells with low expression of lncRNA-UCA1 as recipient cells. Exosomes derived from 5637 cells cultured under normoxic or hypoxic conditions were isolated and identified by transmission electron microscopy, nanoparticle tracking analysis and western blotting analysis. These exosomes were co-cultured with UMUC2 cells to evaluate cell proliferation, migration and invasion. We further investigated the roles of exosomal lncRNA-UCA1 derived from hypoxic 5637 cells by xenograft models. The availability of lncRNA-UCA1 in serum-derived exosomes as a biomarker for bladder cancer was also assessed.ResultsWe found that hypoxic exosomes derived from 5637 cells promoted cell proliferation, migration and invasion, and hypoxic exosomal RNAs could be internalized by three bladder cancer cell lines. Importantly, lncRNA-UCA1 was secreted in hypoxic 5637 cell-derived exosomes. Compared with normoxic exosomes, hypoxic exosomes derived from 5637 cells showed the higher expression levels of lncRNA-UCA1. Moreover, Hypoxic exosomal lncRNA-UCA1 could promote tumor growth and progression though epithelial-mesenchymal transition, in vitro and in vivo. In addition, the expression levels of lncRNA-UCA1 in the human serum-derived exosomes of bladder cancer patients were higher than that in the healthy controls.ConclusionTogether, our results demonstrate that hypoxic bladder cancer cells remodel tumor microenvironment to facilitate tumor growth and development though secreting the oncogenic lncRNA-UCA1-enriched exosomes and exosomal lncRNA-UCA1 in human serum has the possibility as a diagnostic biomarker for bladder cancer.

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Detection of serum long non-coding RNA UCA1 and circular RNAs for the diagnosis of bladder cancer and prediction of recurrence.

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Detection of serum long non-coding RNA UCA1 and circular RNAs for the diagnosis of bladder cancer and prediction of recurrence.

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