Dataset Information

Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays.

ABSTRACT: To estimate the oxidative potential (OP) of particulate matter (PM), two commonly used cell-free, molecular probes were applied: dithiothreitol (DTT) and dichloro-dihydro-fluorescein diacetate (DCFH-DA), and their performance was compared with 9,10-bis (phenylethynyl) anthracene-nitroxide (BPEAnit). To the best of our knowledge, this is the first study in which the performance of the DTT and DCFH has been compared with the BPEAnit probe. The average concentrations of PM, organic carbon (OC) and elemental carbon (EC) for fine (PM2.5) and coarse (PM10) particles were determined. The results were 44.8 ± 13.7, 9.8 ± 5.1 and 9.3 ± 4.8 µg·m-3 for PM2.5 and 75.5 ± 25.1, 16.3 ± 8.7 and 11.8 ± 5.3 µg·m-3 for PM10, respectively, for PM, OC and EC. The water-soluble organic carbon (WSOC) fraction accounted for 42 ± 14% and 28 ± 9% of organic carbon in PM2.5 and PM10, respectively. The average volume normalized OP values for the three assays depended on both the sampling periods and the PM fractions. The OPBPEAnit had its peak at 2 p.m.; in the afternoon, it was three times higher compared to the morning and late afternoon values. The DCFH and BPEAnit results were correlated (r = 0.64), while there was no good agreement between the BPEAnit and the DTT (r = 0.14). The total organic content of PM does not necessarily represent oxidative capacity and it shows varying correlation with the OP. With respect to the two PM fractions studied, the OP was mostly associated with smaller particles.

SUBMITTER: Jovanovic MV

PROVIDER: S-EPMC6950172 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays.

Jovanovic Maja V MV Savic Jasmina Z JZ Salimi Farhad F Stevanovic Svetlana S Brown Reece A RA Jovasevic-Stojanovic Milena M Manojlovic Dragan D Bartonova Alena A Bottle Steven S Ristovski Zoran D ZD

International journal of environmental research and public health 20191205 24

To estimate the oxidative potential (OP) of particulate matter (PM), two commonly used cell-free, molecular probes were applied: dithiothreitol (DTT) and dichloro-dihydro-fluorescein diacetate (DCFH-DA), and their performance was compared with 9,10-bis (phenylethynyl) anthracene-nitroxide (BPEAnit). To the best of our knowledge, this is the first study in which the performance of the DTT and DCFH has been compared with the BPEAnit probe. The average concentrations of PM, organic carbon (OC) and ...[more]

PMID: 31817307

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Project description:Background and Objectives: The oxidative potential (OP) of particulate matter (PM) in cell-free/abiotic systems have been suggested as a possible measure of their biological reactivity and a relevant exposure metric for ambient air PM in epidemiological studies. The present review examined whether the OP of particles correlate with their biological effects, to determine the relevance of these cell-free assays as predictors of particle toxicity. Methods: PubMed, Google Scholar and Web of Science databases were searched to identify relevant studies published up to May 2019. The main inclusion criteria used for the selection of studies were that they should contain (1) multiple PM types or samples, (2) assessment of oxidative potential in cell-free systems and (3) assessment of biological effects in cells, animals or humans. Results: In total, 50 independent studies were identified assessing both OP and biological effects of ambient air PM or combustion particles such as diesel exhaust and wood smoke particles: 32 in vitro or in vivo studies exploring effects in cells or animals, and 18 clinical or epidemiological studies exploring effects in humans. Of these, 29 studies assessed the association between OP and biological effects by statistical analysis: 10 studies reported that at least one OP measure was statistically significantly associated with all endpoints examined, 12 studies reported that at least one OP measure was significantly associated with at least one effect outcome, while seven studies reported no significant correlation/association between any OP measures and any biological effects. The overall assessment revealed considerable variability in reported association between individual OP assays and specific outcomes, but evidence of positive association between intracellular ROS, oxidative damage and antioxidant response in vitro, and between OP assessed by the dithiothreitol (DDT) assay and asthma/wheeze in humans. There was little support for consistent association between OP and any other outcome assessed, either due to repeated lack of statistical association, variability in reported findings or limited numbers of available studies. Conclusions: Current assays for OP in cell-free/abiotic systems appear to have limited value in predicting PM toxicity. Clarifying the underlying causes may be important for further advancement in the field.

Project description:In this study, we examine the oxidative potential of airborne particulate matter (PM) in Beirut, Lebanon, as influenced by dust events originating in the Sahara and Arabian deserts. Segregated fine (< 2.5 ?m) and coarse (2.5-10 ?m) PM samples collected during dust events, as well as during non-dust periods, were analyzed for chemical composition, and the in vitro alveolar macrophage (AM) assay was utilized to determine the oxidative potential of both types of samples. We performed Spearman rank-order correlation analysis between individual chemical components and the oxidative potential of PM to examine the impact of the changes in PM chemical composition due to the occurrence of dust events on overall PM oxidative potential. Our findings revealed that the oxidative potential of Beirut's urban PM during non-dust periods was much higher than during dust episodes for fine PM. Our findings also indicated that tracers of tailpipe emissions (i.e., elemental (EC) and organic carbon (OC)), non-tailpipe emissions (i.e., heavy metals including Cu, Zn, As, Cd, and Pb), and secondary organic aerosols (SOA) (i.e., water-soluble organic carbon, WSOC) were significantly associated with the oxidative potential of PM during dust days and non-dust periods. However, the contribution of desert dust aerosols to Beirut's indigenous PM composition did not exacerbate its oxidative potential, as indicated by the negative correlations between the oxidative potential of PM and the concentrations of crustal elements that were enriched during the dust days. This suggests that aerosols generated during Saharan and Arabian dust events pose no additional health risk to the population due to PM-triggered reactive oxygen species formation. These results significantly contribute to our understanding of the effects of desert dust aerosols on the composition and oxidative potential of PM in several countries throughout the entire Middle East region that are impacted by dust events in the Sahara and Arabian deserts.

Project description:The main objective of this study was chemical characterization and source apportionment of the oxidative potential of ambient PM2.5 samples collected in an urban background area in Athens, Greece. Ambient PM2.5 samples were collected during the summer (June-September) of 2017 and winter (February-March) of 2018 at a residential, urban background site in the outlying neighborhood of the Demokritos National Laboratory in Athens, Greece. The collected PM samples were analyzed for their chemical constituents including metals and trace elements, water-soluble organic carbon (WSOC), elemental and organic carbon (EC/OC), and marker of biomass burning (i.e., levoglucosan). In addition, the DCFH in vitro assay was performed to determine the oxidative potential of the PM2.5 samples. We performed a series of statistical analyses, including Spearman rank-order correlation analysis, principal component analysis (PCA), and multi linear regression (MLR) to determine the most significant species (as source tracers) contributing to the oxidative potential of PM2.5. Our findings revealed that the intrinsic (per PM mass) and extrinsic (per m3 of air volume) oxidative potentials of the collected ambient PM2.5 samples were significantly higher than those measured in many urban areas around the world. The results of the MLR analyses indicated that the major pollution sources contributing to the oxidative potential of ambient PM2.5 were vehicular emissions (characterized by EC) (44%), followed by secondary organic aerosol (SOA) formation (characterized by WSOC) (16%), and biomass burning (characterized by levoglucosan) (9%). The oxidative potential of the collected ambient PM2.5 samples was also higher in summer compared to the winter, mainly due to higher concentrations of EC and WSOC during this season. Results from this study corroborate the impact of traffic and SOA on the oxidative potential of ambient PM2.5 in greater Athens area, and can be helpful in adopting appropriate public health policies regarding detrimental outcomes of exposure to PM2.5.

Dataset Information

Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays.

Publications

Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays.

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