Dataset Information

ASCOT identifies key regulators of neuronal subtype-specific splicing.

ABSTRACT: Public archives of next-generation sequencing data are growing exponentially, but the difficulty of marshaling this data has led to its underutilization by scientists. Here, we present ASCOT, a resource that uses annotation-free methods to rapidly analyze and visualize splice variants across tens of thousands of bulk and single-cell data sets in the public archive. To demonstrate the utility of ASCOT, we identify novel cell type-specific alternative exons across the nervous system and leverage ENCODE and GTEx data sets to study the unique splicing of photoreceptors. We find that PTBP1 knockdown and MSI1 and PCBP2 overexpression are sufficient to activate many photoreceptor-specific exons in HepG2 liver cancer cells. This work demonstrates how large-scale analysis of public RNA-Seq data sets can yield key insights into cell type-specific control of RNA splicing and underscores the importance of considering both annotated and unannotated splicing events.

SUBMITTER: Ling JP

PROVIDER: S-EPMC6952364 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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ASCOT identifies key regulators of neuronal subtype-specific splicing.

Ling Jonathan P JP Wilks Christopher C Charles Rone R Leavey Patrick J PJ Ghosh Devlina D Jiang Lizhi L Santiago Clayton P CP Pang Bo B Venkataraman Anand A Clark Brian S BS Nellore Abhinav A Langmead Ben B Blackshaw Seth S

Nature communications 20200109 1

Public archives of next-generation sequencing data are growing exponentially, but the difficulty of marshaling this data has led to its underutilization by scientists. Here, we present ASCOT, a resource that uses annotation-free methods to rapidly analyze and visualize splice variants across tens of thousands of bulk and single-cell data sets in the public archive. To demonstrate the utility of ASCOT, we identify novel cell type-specific alternative exons across the nervous system and leverage E ...[more]

PMID: 31919425

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ASCOT identifies key regulators of neuronal subtype-specific splicing.

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ASCOT identifies key regulators of neuronal subtype-specific splicing.

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