Ontology highlight
ABSTRACT:
SUBMITTER: Nieto B
PROVIDER: S-EPMC6952385 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Nieto Blanca B Gaspar Sonia G SG Moriggi Giulia G Pestov Dimitri G DG Bustelo Xosé R XR Dosil Mercedes M
Nature communications 20200109 1
Technical problems intrinsic to the purification of preribosome intermediates have limited our understanding of ribosome biosynthesis in humans. Addressing this issue is important given the implication of this biological process in human disease. Here we report a preribosome purification and tagging strategy that overcomes some of the existing technical difficulties. Using these tools, we find that the pre-40S precursors go through two distinct maturation phases inside the nucleolus and follow a ...[more]