Dataset Information

Draft Genome Sequence of Lactobacillus crispatus CIP 104459, Isolated from a Vaginal Swab.

ABSTRACT: We report the draft genome sequence of Lactobacillus crispatus CIP 104459, isolated from a human vaginal swab. This draft genome consists of 1,993,673?bp, with 36.8% G+C content and 2,024 predicted protein-encoding sequences.

SUBMITTER: Clabaut M

PROVIDER: S-EPMC6952664 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Draft Genome Sequence of Lactobacillus crispatus CIP 104459, Isolated from a Vaginal Swab.

Clabaut Maximilien M Boukerb Amine M AM Racine Pierre-Jean PJ Pichon Chantal C Kremser Coralie C Picot Jean-Pierre JP Karsybayeva Madina M Redziniak Gérard G Chevalier Sylvie S Feuilloley Marc G J MGJ

Microbiology resource announcements 20200109 2

We report the draft genome sequence of <i>Lactobacillus crispatus</i> CIP 104459, isolated from a human vaginal swab. This draft genome consists of 1,993,673 bp, with 36.8% G+C content and 2,024 predicted protein-encoding sequences. ...[more]

PMID: 31919178

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Project description:BackgroundLactobacillus crispatus is the dominant species in the vaginal microbiota associated with health and considered a homeostasis biomarker. Interestingly, some strains are even used as probiotics. However, the genetic mechanisms of L. crispatus involved in the control of the vaginal microbiome and protection against bacterial vaginosis (BV) are not entirely known. To further investigate these mechanisms, we sequenced and characterized the first four L. crispatus genomes from vaginal samples from Brazilian women and used genome-wide association study (GWAS) and comparative analyses to identify genetic mechanisms involved in healthy or BV conditions and selective pressures acting in the vaginal microbiome.MethodsThe four genomes were sequenced, assembled using ten different strategies and automatically annotated. The functional characterization was performed by bioinformatics tools comparing with known probiotic strains. Moreover, it was selected one representative strain (L. crispatus CRI4) for in vitro detection of phages by electron microscopy. Evolutionary analysis, including phylogeny, GWAS and positive selection were performed using 46 public genomes strains representing health and BV conditions.ResultsGenes involved in probiotic effects such as lactic acid production, hydrogen peroxide, bacteriocins, and adhesin were identified. Three hemolysins and putrescine production were predicted, although these features are also present in other probiotic strains. The four genomes presented no plasmids, but 14 known families insertion sequences and several prophages were detected. However, none of the mobile genetic elements contained antimicrobial resistance genes. The genomes harbor a CRISPR-Cas subtype II-A system that is probably inactivated due to fragmentation of the genes csn2 and cas9. No genomic feature was associated with a health condition, perhaps due to its multifactorial characteristic. Five genes were identified as under positive selection, but the selective pressure remains to be discovered. In conclusion, the Brazilian strains investigated in this study present potential protective properties, although in vitro and in vivo studies are required to confirm their efficacy and safety to be considered for human use.

Dataset Information

Draft Genome Sequence of Lactobacillus crispatus CIP 104459, Isolated from a Vaginal Swab.

Publications

Draft Genome Sequence of Lactobacillus crispatus CIP 104459, Isolated from a Vaginal Swab.

Similar Datasets

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