Ontology highlight
ABSTRACT:
SUBMITTER: Ligasova A
PROVIDER: S-EPMC6952905 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Ligasová Anna A Vydržalová Markéta M Buriánová Renata R Brůčková Lenka L Večeřová Renata R Janošťáková Anna A Koberna Karel K
Cells 20191125 12
Contamination of cell cultures by mycoplasmas is a very common phenomenon. As they can substantially alter cell metabolism and potentially spread to all cell cultures in laboratory, their early detection is necessary. One of the fastest and cheapest methods of mycoplasma detection relies on the direct staining of mycoplasmas' DNA by DAPI or Hoechst dyes. Although this method is easy and fast to perform, it suffers from the low signal provided by these dyes compared to the nuclear DNA. Therefore, ...[more]