DNA extraction approaches substantially influence the assessment of the human breast milk microbiome.
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ABSTRACT:
SUBMITTER: Douglas CA
PROVIDER: S-EPMC6954186 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
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DNA extraction approaches substantially influence the assessment of the human breast milk microbiome.
Douglas Chloe A CA Ivey Kerry L KL Papanicolas Lito E LE Best Karen P KP Muhlhausler Beverly S BS Rogers Geraint B GB
Scientific reports 20200110 1
In addition to providing nutritional and bioactive factors necessary for infant development, human breast milk contains bacteria that contribute to the establishment of commensal microbiota in the infant. However, the composition of this bacterial community differs considerably between studies. We hypothesised that bacterial DNA extraction methodology from breast milk samples are a substantial contributor to these inter-study differences. We tested this hypothesis by applying five widely employe ...[more]
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