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Non-invasive optical control of endogenous Ca²⁺ channels in awake mice.

ABSTRACT: Optogenetic approaches for controlling Ca²⁺ channels provide powerful means for modulating diverse Ca²⁺-specific biological events in space and time. However, blue light-responsive photoreceptors are, in principle, considered inadequate for deep tissue stimulation unless accompanied by optic fiber insertion. Here, we present an ultra-light-sensitive optogenetic Ca²⁺ modulator, named monSTIM1 encompassing engineered cryptochrome2 for manipulating Ca²⁺ signaling in the brain of awake mice through non-invasive light delivery. Activation of monSTIM1 in either excitatory neurons or astrocytes of mice brain is able to induce Ca²⁺-dependent gene expression without any mechanical damage in the brain. Furthermore, we demonstrate that non-invasive Ca²⁺ modulation in neurons can be sufficiently and effectively translated into changes in behavioral phenotypes of awake mice.

SUBMITTER: Kim S

PROVIDER: S-EPMC6954201 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Non-invasive optical control of endogenous Ca2+ channels in awake mice.

Kim Sungsoo S Kyung Taeyoon T Chung Jae-Hee JH Kim Nury N Keum Sehoon S Lee Jinsu J Park Hyerim H Kim Ho Min HM Lee Sangkyu S Shin Hee-Sup HS Heo Won Do WD

Nature communications 20200110 1

Optogenetic approaches for controlling Ca2+ channels provide powerful means for modulating diverse Ca2+-specific biological events in space and time. However, blue light-responsive photoreceptors are, in principle, considered inadequate for deep tissue stimulation unless accompanied by optic fiber insertion. Here, we present an ultra-light-sensitive optogenetic Ca2+ modulator, named monSTIM1 encompassing engineered cryptochrome2 for manipulating Ca2+ s ...[more]

PMID: 31924789

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