Ontology highlight
ABSTRACT:
SUBMITTER: Mithal A
PROVIDER: S-EPMC6954238 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Mithal Aditya A Capilla Amalia A Heinze Dar D Berical Andrew A Villacorta-Martin Carlos C Vedaie Marall M Jacob Anjali A Abo Kristine K Szymaniak Aleksander A Peasley Megan M Stuffer Alexander A Mahoney John J Kotton Darrell N DN Hawkins Finn F Mostoslavsky Gustavo G
Nature communications 20200110 1
Efficient generation of human induced pluripotent stem cell (hiPSC)-derived human intestinal organoids (HIOs) would facilitate the development of in vitro models for a variety of diseases that affect the gastrointestinal tract, such as inflammatory bowel disease or Cystic Fibrosis. Here, we report a directed differentiation protocol for the generation of mesenchyme-free HIOs that can be primed towards more colonic or proximal intestinal lineages in serum-free defined conditions. Using a CDX2<sup ...[more]