Ontology highlight
ABSTRACT:
SUBMITTER: Askary A
PROVIDER: S-EPMC6954335 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Askary Amjad A Sanchez-Guardado Luis L Linton James M JM Chadly Duncan M DM Budde Mark W MW Cai Long L Lois Carlos C Elowitz Michael B MB
Nature biotechnology 20191118 1
Molecular barcoding technologies that uniquely identify single cells are hampered by limitations in barcode measurement. Readout by sequencing does not preserve the spatial organization of cells in tissues, whereas imaging methods preserve spatial structure but are less sensitive to barcode sequence. Here we introduce a system for image-based readout of short (20-base-pair) DNA barcodes. In this system, called Zombie, phage RNA polymerases transcribe engineered barcodes in fixed cells. The resul ...[more]