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Radioiodinated Nitroxide Derivative for the Detection of Lipid Radicals.

ABSTRACT: Thus far, no accurate measurement technology has been developed to detect lipid alkyl radicals (lipid radicals), which cause lipid peroxidation. Therefore, we aimed to develop a nuclear medical imaging probe that can be taken up in the lipophilic site in cells such as biological membranes, by reacting specifically with the lipid radicals generated there. We designed and synthesized 4-(4-[125I]iodobenzamido)-2,2,6,6-tetramethylpiperidine-1-oxyl, which shows high reactivity to lipid radicals with a high radiochemical yield and purity. Intracellular retention was found to increase significantly when lipid radicals were produced.

SUBMITTER: Yamasaki T

PROVIDER: S-EPMC6956360 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Radioiodinated Nitroxide Derivative for the Detection of Lipid Radicals.

Yamasaki Toshihide T Azuma Risa R Sano Kohei K Munekane Masayuki M Matsuoka Yuta Y Yamada Ken-Ichi KI Mukai Takahiro T

ACS medicinal chemistry letters 20191203 1

Thus far, no accurate measurement technology has been developed to detect lipid alkyl radicals (lipid radicals), which cause lipid peroxidation. Therefore, we aimed to develop a nuclear medical imaging probe that can be taken up in the lipophilic site in cells such as biological membranes, by reacting specifically with the lipid radicals generated there. We designed and synthesized 4-(4-[<sup>125</sup>I]iodobenzamido)-2,2,6,6-tetramethylpiperidine-1-oxyl, which shows high reactivity to lipid rad ...[more]

PMID: 31938462

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