Ontology highlight
ABSTRACT:
SUBMITTER: Garcia-Rodriguez G
PROVIDER: S-EPMC6957109 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Garcia-Rodriguez Gabriela G Talavera Perez Ariel A Konijnenberg Albert A Sobott Frank F Michiels Jan J Loris Remy R
Acta crystallographica. Section F, Structural biology communications 20200101 Pt 1
The Escherichia coli rnlAB operon encodes a toxin-antitoxin module that is involved in protection against infection by bacteriophage T4. The full-length RnlA-RnlB toxin-antitoxin complex as well as the toxin RnlA were purified to homogeneity and crystallized. When the affinity tag is placed on RnlA, RnlB is largely lost during purification and the resulting crystals exclusively comprise RnlA. A homogeneous preparation of RnlA-RnlB containing stoichiometric amounts of both proteins could only be ...[more]