Differential gene expression profile analysis in corticosterone-treated PC12 cells.
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ABSTRACT:
SUBMITTER: Zhao JJ
PROVIDER: S-EPMC6958082 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
Differential gene expression profile analysis in corticosterone-treated PC12 cells.
Zhao Jing-Jie JJ Zhang Ping P Li Li L Chen Shu-Xing SX Joines Allison A Wu Donald D Wong Megan M Shahan Justin J Golden Teresa T Wu Ning N Li Ming-Zhen MZ
International journal of clinical and experimental pathology 20180601 6
Major depressive disorder (MDD) is a highly prevalent psychiatric disorder that has been ranked as the 4th leading cause of disability worldwide. Past clinical and laboratory evidence has confirmed that abnormalities of the hypothalamic-pituitary-adrenal (HPA)-axis are involved in MDD development. In this study, we took advantage of corticosterone treatment of PC12 cells as a model to identify genes regulated by HPA-axis hormones. Next-generation RNA-Seq technology was utilized to explore genome ...[more]
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Project description:BackgroundMechanisms under immune response against Candida albicans (C. albicans) remain largely unknown. To better understand the mechanisms of innate immune response against C. albicans, we analyzed the gene expression profile of THP-1 cells stimulated with heat-killed C. albicans.MethodsTHP-1 cells were stimulated with heat-killed C. albicans for 9 hours at a ratio of 1:1, and gene expression profile of the cells was analyzed using Whole Human Genome Oligo Microarray. Differentially expressed genes were defined as change folds more than 2 and with statistical significance. Gene ontology (GO) and pathway analysis were used to systematically identify biological connections of differentially expressed genes, as well as the pathways associated with the immune response against C. albicans.ResultsA total of 355 genes were up-regulated and 715 genes were down-regulated significantly. The up-regulated genes were particularly involved in biological process of RNA processing and pathway of the spliceosome. In case of down-regulated genes, the particularly involved immune-related pathways were G-protein coupled receptor signaling pathway, calcium signaling pathway, MAPK signaling pathway and Ras pathway.ConclusionsWe depict the gene expression profile of heat-killed C. albicans stimulated THP-1 cells, and identify the major pathways involved in immune response against C. albicans. These pathways are potential candidate targets for developing anti-C. albicans agent.
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