CaMKII versus DAPK1 Binding to GluN2B in Ischemic Neuronal Cell Death after Resuscitation from Cardiac Arrest.
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ABSTRACT:
SUBMITTER: Buonarati OR
PROVIDER: S-EPMC6959131 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Publications
CaMKII versus DAPK1 Binding to GluN2B in Ischemic Neuronal Cell Death after Resuscitation from Cardiac Arrest.
Buonarati Olivia R OR Cook Sarah G SG Goodell Dayton J DJ Chalmers Nicholas E NE Rumian Nicole L NL Tullis Jonathan E JE Restrepo Susana S Coultrap Steven J SJ Quillinan Nidia N Herson Paco S PS Bayer K Ulrich KU
Cell reports 20200101 1
DAPK1 binding to GluN2B was prominently reported to mediate ischemic cell death in vivo. DAPK1 and CaMKII bind to the same GluN2B region, and their binding is mutually exclusive. Here, we show that mutating the binding region on GluN2B (L1298A/R1300Q) protected against neuronal cell death induced by cardiac arrest followed by resuscitation. Importantly, the GluN2B mutation selectively abolished only CaMKII, but not DAPK1, binding. During ischemic or excitotoxic insults, CaMKII further accumulate ...[more]
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