Ontology highlight
ABSTRACT:
SUBMITTER: Shen J
PROVIDER: S-EPMC6959245 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Nature communications 20200114 1
The ability to detect low numbers of microbial cells in food and clinical samples is highly valuable but remains a challenge. Here we present a detection system (called 'APC-Cas') that can detect very low numbers of a bacterial pathogen without isolation, using a three-stage amplification to generate powerful fluorescence signals. APC-Cas involves a combination of nucleic acid-based allosteric probes and CRISPR-Cas13a components. It can selectively and sensitively quantify Salmonella Enteritidis ...[more]